Open Access

Serum metabolomic profile in genetically modified cows carrying human α‑lactalbumin gene

  • Authors:
    • Qin Wang
    • Wei Fu
    • Xiaofei Liu
    • Jianwu Wang
    • Chunyan Feng
    • Songyin Qiu
    • Xiaolin Li
    • Dandan Liu
    • Shuifang Zhu
    • Xiangmei Lin
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/mmr.2017.7768
  • Pages:8833-8841
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the serum metabolomic profiles in genetically modified cows carrying and expressing human lactalbumin α (LALBA) and non‑LALBA cows, and identify altered metabolic characteristics following the genetic modification. Serum biochemistry indexes were measured according to protocols recommended by International Federation of Clinical Chemistry. The metabolomic profiles were determined using the serum samples collected from LALBA (n=6) and non‑LALBA cows (n=6). Welch's two‑sample t‑test was used to identify the metabolites that significantly differed between the LALBA and non‑LALBA groups (fold‑change ≠ 1 and P<0.05), followed by random forest and pathway analysis. The serum biochemistry indexes of LALBA and non‑LALBA cows were within the normal ranges of healthy cows. A total of 273 metabolites were detected, among which 79 metabolites, including 46 increased and 33 decreased metabolites, differed significantly between the LALBA and non‑LALBA groups. Random forest analysis identified 30 potential key metabolites, including 14 elevated and 16 reduced metabolites. These metabolites were primarily involved in pathways concerning the metabolism of leucine, isoleucine, valine, tryptophan and lipids, such as myristate and eicosapentaenoate. However, the serum in LALBA cow had unique metabolomic signature compared with non‑LALBA cows. The accumulation of polyunsaturated fatty acids and amino acids, and the reduced levels of long chain saturated fatty acids in serum may benefit LALBA cows. However, further investigations are required to validate these benefits and the corresponding mechanisms.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Wang, Q., Fu, W., Liu, X., Wang, J., Feng, C., Qiu, S. ... Lin, X. (2017). Serum metabolomic profile in genetically modified cows carrying human α‑lactalbumin gene. Molecular Medicine Reports, 16, 8833-8841. https://doi.org/10.3892/mmr.2017.7768
MLA
Wang, Q., Fu, W., Liu, X., Wang, J., Feng, C., Qiu, S., Li, X., Liu, D., Zhu, S., Lin, X."Serum metabolomic profile in genetically modified cows carrying human α‑lactalbumin gene". Molecular Medicine Reports 16.6 (2017): 8833-8841.
Chicago
Wang, Q., Fu, W., Liu, X., Wang, J., Feng, C., Qiu, S., Li, X., Liu, D., Zhu, S., Lin, X."Serum metabolomic profile in genetically modified cows carrying human α‑lactalbumin gene". Molecular Medicine Reports 16, no. 6 (2017): 8833-8841. https://doi.org/10.3892/mmr.2017.7768