Open Access

Matrine in association with FD‑2 stimulates F508del‑cystic fibrosis transmembrane conductance regulator activity in the presence of corrector VX809

  • Authors:
    • Barbara Marengo
    • Andrea Speciale
    • Lisa Senatore
    • Silvano Garibaldi
    • Francesca Musumeci
    • Erika Nieddu
    • Benedetta Pollarolo
    • Maria Adelaide Pronzato
    • Silvia Schenone
    • Mauro Mazzei
    • Cinzia Domenicotti
  • View Affiliations

  • Published online on: October 6, 2017     https://doi.org/10.3892/mmr.2017.7736
  • Pages:8849-8853
  • Copyright: © Marengo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: PDF 0 views | HTML 0 views
0

Abstract

Cystic fibrosis is caused by mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, and the predominant mutation is termed Phe508del (F508del). Therapy for F508del‑CFTR patients is based on the use of Orkambi®, a combination of VX809 and VX770. However, though Orkambi leads to an improvement in the lung function of patients, a progressive reduction in its efficacy has been observed. In order to overcome this effect, the aim of the present study was to investigate the role of matrine and the in‑house compound FD‑2 in increasing the action of VX809 and VX770. Fischer rat thyroid cells overexpressing F508del‑CFTR were treated with matrine, VX809 (corrector) and/or with a number of potentiators (VX770, FD‑1 and FD‑2). The results demonstrated that matrine was able to stimulate CFTR activity and, in association with FD‑2, increased the functionality of the channel in the presence of VX809. Based on these results, it may be hypothesized that FD‑2 may be a novel and more effective potentiator compared with VX770.

Related Articles

Journal Cover

December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Marengo, B., Speciale, A., Senatore, L., Garibaldi, S., Musumeci, F., Nieddu, E. ... Domenicotti, C. (2017). Matrine in association with FD‑2 stimulates F508del‑cystic fibrosis transmembrane conductance regulator activity in the presence of corrector VX809. Molecular Medicine Reports, 16, 8849-8853. https://doi.org/10.3892/mmr.2017.7736
MLA
Marengo, B., Speciale, A., Senatore, L., Garibaldi, S., Musumeci, F., Nieddu, E., Pollarolo, B., Pronzato, M. A., Schenone, S., Mazzei, M., Domenicotti, C."Matrine in association with FD‑2 stimulates F508del‑cystic fibrosis transmembrane conductance regulator activity in the presence of corrector VX809". Molecular Medicine Reports 16.6 (2017): 8849-8853.
Chicago
Marengo, B., Speciale, A., Senatore, L., Garibaldi, S., Musumeci, F., Nieddu, E., Pollarolo, B., Pronzato, M. A., Schenone, S., Mazzei, M., Domenicotti, C."Matrine in association with FD‑2 stimulates F508del‑cystic fibrosis transmembrane conductance regulator activity in the presence of corrector VX809". Molecular Medicine Reports 16, no. 6 (2017): 8849-8853. https://doi.org/10.3892/mmr.2017.7736