Impact of biopersistent fibrous dusts on glycolysis, glutaminolysis and serine metabolism in A549 cells
- Sybille Wache
- Simone Helmig
- Dirk Walter
- Joachim Schneider
- Sybille Mazurek
Published online on: October 5, 2017
The conversion rates of different metabolic pathways summarized as a metabolic signature mirror the physiological functions and the general physiological status of a cell. The present study compared the impact of crocidolite and chrysotile asbestos, glass fibers and multi‑walled carbon nanotubes (MWCN) of two different lengths (1‑2 µm and 5‑15 µm) on the conversion rates in glycolysis, glutaminolysis and serine metabolism of A549 cells. The concentration tested was 1 µg/cm2 for all fibers. A concentration of 5 µg/cm2 was additionally used for chrysotile and crocidolite, and 25 µg/cm2 for glass fibers and MWCN. With respect to the inhibitory effect on cell proliferation and the extent of metabolic alterations, the present study revealed the following ranking among the fibers tested: Chrysotile>crocidolite>glass fibers>MWCN 5‑15 µm>MWCN 1‑2 µm. For the asbestos and glass fibers this ranking correlated best with the number of fibers. It appeared that the results observed for MWCN did not match this correlation. However, electron microscopy revealed an agglomeration of MWCN. The agglomeration decreased the toxicologically relevant number of fibers by forming larger particle‑like shapes and explained the smaller effects of MWCN 5‑15 µm and 1‑2 µm on cell proliferation and metabolism.