The FNR modules contribute to control nitric oxide synthase catalysis revealed by chimera enzymes

  • Authors:
    • Ruiqiang Wang
    • Biyue Wang
    • Bowen Zheng
    • Pupu Ma
    • Rong Gou
    • Yuanyuan Guo
    • Fengmei Chen
    • Hua Li
    • Yi Wang
    • Juanjuan Pu
    • Lin Tang
  • View Affiliations

  • Published online on: October 9, 2017     https://doi.org/10.3892/mmr.2017.7745
  • Pages:9263-9269
Metrics: PDF 0 views | HTML 0 views
0

Abstract

The reductase domains of neuronal NOS, endothelial NOS and two constitutive nitric oxide synthase (cNOS) share higher sequence similarity (>60%). In order to evaluate the role of ferredoxin‑NADP+ reductase (FNR) module in adjusting NOS catalytic activities, chimeras were by interchanging the FNR‑like module between endothelial NOS and neuronal NOS in the present study. The assays of steady‑state enzymatic activities for cytochrome c and ferricyanide reduction, NO synthesis and NADPH oxidation were performed spectrophotometrically. The two NOS FNR modules transferred their ferricyanide reductase character to the chimera enzymes. Results showed that the FNR module was important in adjusting electrons flow through the reductase domain and out of the FMN module. Results indicated that the FNR module was critical in controlling the electron transfer capacities of the FMN module.

Related Articles

Journal Cover

December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wang, R., Wang, B., Zheng, B., Ma, P., Gou, R., Guo, Y. ... Tang, L. (2017). The FNR modules contribute to control nitric oxide synthase catalysis revealed by chimera enzymes. Molecular Medicine Reports, 16, 9263-9269. https://doi.org/10.3892/mmr.2017.7745
MLA
Wang, R., Wang, B., Zheng, B., Ma, P., Gou, R., Guo, Y., Chen, F., Li, H., Wang, Y., Pu, J., Tang, L."The FNR modules contribute to control nitric oxide synthase catalysis revealed by chimera enzymes". Molecular Medicine Reports 16.6 (2017): 9263-9269.
Chicago
Wang, R., Wang, B., Zheng, B., Ma, P., Gou, R., Guo, Y., Chen, F., Li, H., Wang, Y., Pu, J., Tang, L."The FNR modules contribute to control nitric oxide synthase catalysis revealed by chimera enzymes". Molecular Medicine Reports 16, no. 6 (2017): 9263-9269. https://doi.org/10.3892/mmr.2017.7745