miRNA‑27a regulates arthritis via PPARγ in vivo and in vitro

  • Authors:
    • Yu Xiao
    • Bing Li
    • Jun Liu
  • View Affiliations

  • Published online on: January 31, 2018     https://doi.org/10.3892/mmr.2018.8531
  • Pages: 5454-5462
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study investigated the role of microRNA (miR)‑27a in the development of arthritis and its mechanism of action. Initially, collagen was used to develop an in vivo rat model of arthritis. Changes in the miRs in the rats were analyzed. It was subsequently observed that miR‑27a expression was reduced in patients with arthritis, compared with the control group. In the present study an in vitro miR‑27a overexpression model of arthritis was established and it was observed that miR‑27a increased the proliferation of osteoblast‑like cells in vitro. miR‑27a overexpression promoted osteogenic differentiation, increased alkaline phosphatase (ALP) and osteoporosis (OST) content, induced insulin‑like growth factor binding protein-5 (IGFBP‑5) protein expression, reduced inflammation and suppressed peroxisome proliferator‑activated receptor γ (PPARγ) and matrix metalloproteinase-17 (MMP‑17) protein expression in arthritis. However, miR‑27a downregulation inhibited osteogenic differentiation, increased inflammation and PPARγ and MMP‑17 protein expression and suppressed ALP and OST content in an in vitro model of arthritis. The PPARγ inhibitor reduced the function of miR‑27a downregulation on arthritis. Therefore the results of the present study revealed that miR‑27a regulates arthritis via PPARγ.
View Figures
View References

Related Articles

Journal Cover

April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiao Y, Li B and Liu J: miRNA‑27a regulates arthritis via PPARγ in vivo and in vitro. Mol Med Rep 17: 5454-5462, 2018.
APA
Xiao, Y., Li, B., & Liu, J. (2018). miRNA‑27a regulates arthritis via PPARγ in vivo and in vitro. Molecular Medicine Reports, 17, 5454-5462. https://doi.org/10.3892/mmr.2018.8531
MLA
Xiao, Y., Li, B., Liu, J."miRNA‑27a regulates arthritis via PPARγ in vivo and in vitro". Molecular Medicine Reports 17.4 (2018): 5454-5462.
Chicago
Xiao, Y., Li, B., Liu, J."miRNA‑27a regulates arthritis via PPARγ in vivo and in vitro". Molecular Medicine Reports 17, no. 4 (2018): 5454-5462. https://doi.org/10.3892/mmr.2018.8531