Open Access

Lentivirus‑mediated MDA7/IL24 expression inhibits the proliferation of hepatocellular carcinoma cells

  • Authors:
    • Chao Ma
    • Ling‑Ling Zhao
    • Heng‑Jun Zhao
    • Jiu‑Wei Cui
    • Wei Li
    • Nan‑Ya Wang
  • View Affiliations

  • Published online on: February 16, 2018     https://doi.org/10.3892/mmr.2018.8616
  • Pages: 5764-5773
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MDA7/IL24 is a member of the IL‑10 gene family that functions as a cytokine. Notably, supra‑physiological endogenous MDA7 levels have been indicated to suppress tumor growth and induce apoptosis in different cancer types. In the present study, MDA7 roles were investigated during the proliferation of hepatocellular carcinoma (HCC) cells and the molecular mechanisms underlying this process. A lentiviral vector expressing MDA7/IL24 (LV‑MDA7/IL24) was constructed and used to infect HCC SMMC‑7721 cells. The expression levels of MDA7/IL24 in these cells were determined using RT‑qPCR and western blot analysis. The effects of LV‑MDA7/IL24 on cell proliferation were analyzed using MTT and colony formation assays. Furthermore, the influence of LV‑MDA7/IL24 on cell apoptosis and cell cycle distribution were detected using flow cytometry. The underlying molecular mechanisms were investigated using microarray and western blot analysis. The expression of MDA7/IL24 was confirmed to be significantly increased in the cells infected with LV‑MDA7/IL24 compared with that the negative‑control infected group. Lentivirus‑mediated MDA7/IL24 expression was found to inhibit HCC cell proliferation and colony formation, and it also induced cell arrest and apoptosis. Microarray analysis and western blotting results indicated that multiple cancer‑associated pathways and oncogenes are regulated by MDA7/IL24, including cell cycle regulatory and apoptosis activation pathway. In conclusion, it was determined that MDA7/IL24 inhibits the proliferation and reduces the tumorigenicity of HCC cells by regulating cell cycle progression and inducing apoptosis, indicating that it may be used as a potential prognostic and therapeutic target in HCC.
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April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ma C, Zhao LL, Zhao HJ, Cui JW, Li W and Wang NY: Lentivirus‑mediated MDA7/IL24 expression inhibits the proliferation of hepatocellular carcinoma cells. Mol Med Rep 17: 5764-5773, 2018
APA
Ma, C., Zhao, L., Zhao, H., Cui, J., Li, W., & Wang, N. (2018). Lentivirus‑mediated MDA7/IL24 expression inhibits the proliferation of hepatocellular carcinoma cells. Molecular Medicine Reports, 17, 5764-5773. https://doi.org/10.3892/mmr.2018.8616
MLA
Ma, C., Zhao, L., Zhao, H., Cui, J., Li, W., Wang, N."Lentivirus‑mediated MDA7/IL24 expression inhibits the proliferation of hepatocellular carcinoma cells". Molecular Medicine Reports 17.4 (2018): 5764-5773.
Chicago
Ma, C., Zhao, L., Zhao, H., Cui, J., Li, W., Wang, N."Lentivirus‑mediated MDA7/IL24 expression inhibits the proliferation of hepatocellular carcinoma cells". Molecular Medicine Reports 17, no. 4 (2018): 5764-5773. https://doi.org/10.3892/mmr.2018.8616