Expression and functional analysis of TCF4 isoforms in human glioma cells

Liu,Zheng; Song,Jian; Wu,Yue; Yao,Shun; Xu,Guo‑Zheng; Diao,Bo

doi:10.3892/mmr.2018.8553

Expression and functional analysis of TCF4 isoforms in human glioma cells

Authors:
- Zheng Liu
- Jian Song
- Yue Wu
- Shun Yao
- Guo‑Zheng Xu
- Bo Diao
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Affiliations: Department of Clinical Medicine, Graduate School of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Neurosurgery, Wuhan General Hospital of PLA, Wuhan, Hubei 430070, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Hubei Key Laboratory of Central Nervous System Tumor and Intervention, Wuhan General Hospital of PLA, Wuhan, Hubei 430070, P.R. China
Published online on: February 2, 2018 https://doi.org/10.3892/mmr.2018.8553
Pages: 6023-6027

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Abstract

Transcription factor 4 (TCF4) is a member of the T-cell factor/lymphoid enhancer factor (TCF/LEF) transcription factor family in the Wnt/β‑catenin signaling pathway. The alternative splicing of TCF4 has been reported to exhibit potential carcinogenic properties in various cancer types. In the present study, TCF4 isoforms were cloned and identified in three human glioma cell lines, with the majority of splicing regions being exons 4, 5, 14, 15, and 16. Using MTT assays, it was demonstrated that the overexpression of TCF4 isoforms inhibits the proliferation of U251 cells. Flow cytometry and wound healing analyses revealed that the overexpression of TCF4 isoforms induced cell apoptosis and migration. Taken together, the β‑catenin binding domain of the TCF4 isoforms inhibited cell proliferation, and induced cell apoptosis and migration in glioma. Furthermore, all the isoforms identified contained the N‑terminal part of TCF4 including the β‑catenin binding domain. This implied that a high expression of TCF4 isoforms may lead to Wnt/β‑catenin signal activation and potentially promote malignant glioma development.

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