Open Access

Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells

  • Authors:
    • Jian‑Zhong Li
    • Tian‑Hui Cao
    • Jin‑Cheng Han
    • Hui Qu
    • Shuang‑Quan Jiang
    • Bao‑Dong Xie
    • Xiao‑Long Yan
    • Hua Wu
    • Xiang‑Lan Liu
    • Fan Zhang
    • Xiao‑Ping Leng
    • Kai Kang
    • Shu‑Lin Jiang
  • View Affiliations

  • Published online on: February 1, 2019     https://doi.org/10.3892/mmr.2019.9922
  • Pages: 2660-2670
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Adipose‑derived stem cells (ADSCs) and bone marrow‑derived stem cells (BMSCs) are considered to be prospective sources of mesenchymal stromal cells (MSCs), that can be used in cell therapy for atherosclerosis. The present study investigated whether ADSCs co‑cultured with M1 foam macrophages via treatment with oxidized low‑density lipoprotein (ox‑LDL) would lead to similar or improved anti‑inflammatory effects compared with BMSCs. ADSCs, peripheral blood monocytes, BMSCs and ox‑LDL were isolated from ten coronary heart disease (CHD) patients. After three passages, the supernatants of the ADSCs and BMSCs were collected and systematically analysed by liquid chromatography‑quadrupole time‑of‑flight‑mass spectrometry (6530; Agilent Technologies, Inc., Santa Clara, CA, USA). Cis‑9, trans‑11 was deemed to be responsible for the potential differences in the metabolic characteristics of ADSCs and BMSCs. These peripheral blood monocytes were characterized using flow cytometry. Following peripheral blood monocytes differentiation into M1 macrophages, the formation of M1 foam macrophages was achieved through treatment with ox‑LDL. Overall, 2x106 ADSCs, BMSCs or BMSCs+cis‑9, trans‑11 were co‑cultured with M1 foam macrophages. Anti‑inflammatory capability, phagocytic activity, anti‑apoptotic capability and cell viability assays were compared among these groups. It was demonstrated that the accumulation of lipid droplets decreased following ADSCs, BMSCs or BMSCs+cis‑9, trans‑11 treatment in M1 macrophages derived from foam cells. Consistently, ADSCs exhibited great advantageous anti‑inflammatory capabilities, phagocytic activity, anti‑apoptotic capability activity and cell viability over BMSCs or BMSCs+cis‑9, trans‑11. Additionally, BMSCs+cis‑9, trans‑11 also demonstrated marked improvement in anti‑inflammatory capability, phagocytic activity, anti‑apoptotic capability activity and cell viability in comparison with BMSCs. The present results indicated that ADSCs would be more appropriate for transplantation to treat atherosclerosis than BMSCs alone or BMSCs+cis‑9, trans‑11. This may be an important mechanism to regulate macrophage immune function.
View Figures
View References

Related Articles

Journal Cover

April 2019
Volume 19 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Li, J., Cao, T., Han, J., Qu, H., Jiang, S., Xie, B. ... Jiang, S. (2019). Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells. Molecular Medicine Reports, 19, 2660-2670. https://doi.org/10.3892/mmr.2019.9922
MLA
Li, J., Cao, T., Han, J., Qu, H., Jiang, S., Xie, B., Yan, X., Wu, H., Liu, X., Zhang, F., Leng, X., Kang, K., Jiang, S."Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells". Molecular Medicine Reports 19.4 (2019): 2660-2670.
Chicago
Li, J., Cao, T., Han, J., Qu, H., Jiang, S., Xie, B., Yan, X., Wu, H., Liu, X., Zhang, F., Leng, X., Kang, K., Jiang, S."Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells". Molecular Medicine Reports 19, no. 4 (2019): 2660-2670. https://doi.org/10.3892/mmr.2019.9922