MicroRNA profiling of human gastric cancer

  • Authors:
    • Yu Yao
    • Ai-Li Suo
    • Zong-Fang Li
    • Li-Ying Liu
    • Tao Tian
    • Lei Ni
    • Wang-Gang Zhang
    • Ke-Jun Nan
    • Tu-Sheng Song
    • Chen Huang
  • View Affiliations

  • Published online on: Sunday, November 1, 2009
  • Pages: 963-970 DOI: 10.3892/mmr_00000199

Abstract

MicroRNAs are a group of small non-coding RNAs that modulate gene expression. The de-regulation of microRNA expression has been found in several types of cancer. To study the role of microRNAs in gastric cancer (GC), we analyzed the expression profile of 847 microRNAs in GC from Chinese patients. Total RNA was used for hybridization on the miRCURY LNA Array (v. 11.0), which contains probes specific for 847 human microRNAs. The results from the miRNA microarray analysis were validated by real-time RT-PCR. A total of 24 miRNAs with a more than 2-fold change were differentially expressed between normal gastric tissue and GC. Of these, 22 miRNAs (miR-223, miR-106b, miR-147, miR-34a, miR-130b*, miR-106a, miR-18a, miR-17, miR-98, miR-616*, miR-181a-2*, miR-185, miR-1259, miR-601, miR-196a*, miR-221*, miR-302f, miR-340*, miR-337-3p, miR-520c-3p, miR-575 and miR-138) were significantly up-regulated in GC (P<0.05), whereas only miR-638 and miR-378 were significantly down-regulated in GC (P<0.05) compared to normal gastric tissue. The expression of miR-185 and miR-638, as measured by miRNA microarray analysis, was in agreement with the expression level of these microRNAs found by real-time RT-PCR in the same samples. Our results show that microRNAs are de-regulated in GC, suggesting the involvement of these genes in the development and progression of gastric cancer.

Related Articles

Journal Cover

November 2009
Volume 2 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2015 Impact Factor: 1.559
Ranked #48/123 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yao, Y., Suo, A., Li, Z., Liu, L., Tian, T., Ni, L. ... Huang, C. (2009). MicroRNA profiling of human gastric cancer. Molecular Medicine Reports, 2, 963-970. http://dx.doi.org/10.3892/mmr_00000199
MLA
Yao, Y., Suo, A., Li, Z., Liu, L., Tian, T., Ni, L., Zhang, W., Nan, K., Song, T., Huang, C."MicroRNA profiling of human gastric cancer". Molecular Medicine Reports 2.6 (2009): 963-970.
Chicago
Yao, Y., Suo, A., Li, Z., Liu, L., Tian, T., Ni, L., Zhang, W., Nan, K., Song, T., Huang, C."MicroRNA profiling of human gastric cancer". Molecular Medicine Reports 2, no. 6 (2009): 963-970. http://dx.doi.org/10.3892/mmr_00000199