In vitro biological activity of bovine milk ribonuclease-4

  • Authors:
    • R. Di Liddo
    • D. Dalzoppo
    • S. Baiguera
    • M. T. Conconi
    • M. Dettin
    • P. P. Parnigotto
    • C. Grandi
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000229
  • Pages: 127-132
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Abstract

Several members of the ribonuclease superfamily possess a variety of interesting biological properties, including ribonucleolytic, angiogenic, antiproliferative, cytotoxic, embryotoxic, aspermatogenic and antitumoral activity. In this study, we report the purification from bovine milk of a protein with structural and enzymatic properties very similar to those of ribonuclease-4 (RNase-4), which is normally present in the liver and lungs, and examined its functional properties, biological activity and cytotoxic effects. RNase-4, at physiological concentrations, had a positive effect on the vitality and proliferation of human umbilical vein endothelial cells. Moreover, it induced an increase in cellular migration and the formation of in vitro capillary-like structures. We also evaluated the effect of RNase-4 in vitro on human breast, colorectal and cervical carcinoma cell lines. The protein was revealed to have a cytotoxic effect similar to that of RNase-A. We suggest that the positive effects of RNase-4 on normal cells were due to its particularly close interaction with RNase inhibitor, while good conformational stability and resistance to proteolytic degradation potentially favour ribonuclease cytotoxicity.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Di Liddo R, Dalzoppo D, Baiguera S, Conconi MT, Dettin M, Parnigotto PP and Grandi C: In vitro biological activity of bovine milk ribonuclease-4 . Mol Med Rep 3: 127-132, 2010.
APA
Di Liddo, R., Dalzoppo, D., Baiguera, S., Conconi, M.T., Dettin, M., Parnigotto, P.P., & Grandi, C. (2010). In vitro biological activity of bovine milk ribonuclease-4 . Molecular Medicine Reports, 3, 127-132. https://doi.org/10.3892/mmr_00000229
MLA
Di Liddo, R., Dalzoppo, D., Baiguera, S., Conconi, M. T., Dettin, M., Parnigotto, P. P., Grandi, C."In vitro biological activity of bovine milk ribonuclease-4 ". Molecular Medicine Reports 3.1 (2010): 127-132.
Chicago
Di Liddo, R., Dalzoppo, D., Baiguera, S., Conconi, M. T., Dettin, M., Parnigotto, P. P., Grandi, C."In vitro biological activity of bovine milk ribonuclease-4 ". Molecular Medicine Reports 3, no. 1 (2010): 127-132. https://doi.org/10.3892/mmr_00000229