Open Access

CCDC132 is highly expressed in atopic dermatitis T cells

  • Authors:
    • Yoshiko Matsumoto
    • Yukiho Imai
    • Yuji Sugita
    • Toshio Tanaka
    • Gozoh Tsujimoto
    • Hirohisa Saito
    • Tadahilo Oshida
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000222
  • Pages: 83-87
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

The analysis of genes preferentially expressed in the peripheral blood cells of atopic dermatitis patients may provide information on the molecular pathogenesis of the disease. We employed differential display PCR to clone a new gene (AB100163) with 99% homology to coiled-coil domain containing 132, transcript variant 1 (CCDC132) (NM_017667) (aliases, FLJ20097, FLJ23581, KIAA1861 and MGC176659). Full-length CCDC132 of approximately 4 kbp encodes mRNA expressed in almost all tissues, in particular brain tissue and skeletal muscle. A homologous gene has also been identified in mice. Using Western blot analysis, 111 kDa CCDC132 protein was detected in two human T-cell lines, MOLT-4 and Jurkat, and in the human cervical adenocarcinoma cell line HeLa. Quantitative RT-PCR revealed transcription levels of CCDC132 in the T cells of atopic dermatitis patients to be higher than in those of normal individuals. This suggests that changes in CCDC132 expression may be involved in the course of atopic dermatitis.

Related Articles

Journal Cover

January 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Matsumoto, Y., Imai, Y., Sugita, Y., Tanaka, T., Tsujimoto, G., Saito, H., & Oshida, T. (2010). CCDC132 is highly expressed in atopic dermatitis T cells . Molecular Medicine Reports, 3, 83-87. https://doi.org/10.3892/mmr_00000222
MLA
Matsumoto, Y., Imai, Y., Sugita, Y., Tanaka, T., Tsujimoto, G., Saito, H., Oshida, T."CCDC132 is highly expressed in atopic dermatitis T cells ". Molecular Medicine Reports 3.1 (2010): 83-87.
Chicago
Matsumoto, Y., Imai, Y., Sugita, Y., Tanaka, T., Tsujimoto, G., Saito, H., Oshida, T."CCDC132 is highly expressed in atopic dermatitis T cells ". Molecular Medicine Reports 3, no. 1 (2010): 83-87. https://doi.org/10.3892/mmr_00000222