Emodin inhibits extracellular matrix synthesis by suppressing p38 and ERK1/2 pathways in TGF-β1-stimulated NRK-49F cells

  • Authors:
    • Bin Zhu
    • Yi Lin
    • Cai-Feng Zhu
    • Xiao-Ling Zhu
    • Chen-Zhao Huang
    • Ying Lu
    • Xiao-Xia Cheng
    • Yong-Jun Wang
  • View Affiliations

  • Published online on: February 28, 2011     https://doi.org/10.3892/mmr.2011.444
  • Pages: 505-509
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Abstract

Emodin has been demonstrated to inhibit the fibrotic process in chronic renal disease, but its mechanisms have yet to be fully elucidated. This study was carried out to investigate the effects of emodin on extracellular matrix (ECM) synthesis in TGF-β1-stimulated NRK-49F cells. NRK-49F cells stimulated with TGF-β1 were incubated with various concentrations of emodin. ECM proteins, including collagen type III and fibronectin, were detected using ELISA. ERK1/2, p38 and JNK phosphorylation were measured by Western blotting. p38, ERK1/2 and JNK were respectively inhibited with the specific inhibitors SB203580, PD98059 and SP600125. Emodin slightly inhibited the expression of fibronectin and collagen type III in NRK-49F cells without TGF-β1 treatment, and significantly suppressed fibronectin and collagen type III secretion in TGF-β1-stimulated NRK-49F cells. ERK1/2 and p38 specific inhibitors, but not JNK inhibitor, suppressed the TGF-β1-induced expression of fibronectin and collagen type III. Our previous study demonstrated that there was no crosstalk between ERK1/2, p38 and JNK signals in TGF-β1-stimulated NRK-49F cells. Here, we found that emodin inhibited the phosphorylation of ERK1/2 and p38 significantly, but did not suppress the phosphorylation of JNK. In summary, emodin suppresses fibronectin and collagen type III expression via the inhibition of ERK1/2 and p38 phosphorylation in TGF-β1-stimulated NRK-49F cells.

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May-June 2011
Volume 4 Issue 3

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Spandidos Publications style
Zhu B, Lin Y, Zhu C, Zhu X, Huang C, Lu Y, Cheng X and Wang Y: Emodin inhibits extracellular matrix synthesis by suppressing p38 and ERK1/2 pathways in TGF-β1-stimulated NRK-49F cells. Mol Med Rep 4: 505-509, 2011
APA
Zhu, B., Lin, Y., Zhu, C., Zhu, X., Huang, C., Lu, Y. ... Wang, Y. (2011). Emodin inhibits extracellular matrix synthesis by suppressing p38 and ERK1/2 pathways in TGF-β1-stimulated NRK-49F cells. Molecular Medicine Reports, 4, 505-509. https://doi.org/10.3892/mmr.2011.444
MLA
Zhu, B., Lin, Y., Zhu, C., Zhu, X., Huang, C., Lu, Y., Cheng, X., Wang, Y."Emodin inhibits extracellular matrix synthesis by suppressing p38 and ERK1/2 pathways in TGF-β1-stimulated NRK-49F cells". Molecular Medicine Reports 4.3 (2011): 505-509.
Chicago
Zhu, B., Lin, Y., Zhu, C., Zhu, X., Huang, C., Lu, Y., Cheng, X., Wang, Y."Emodin inhibits extracellular matrix synthesis by suppressing p38 and ERK1/2 pathways in TGF-β1-stimulated NRK-49F cells". Molecular Medicine Reports 4, no. 3 (2011): 505-509. https://doi.org/10.3892/mmr.2011.444