Potential role of β-elemene on histone H1 in the H22 ascites hepatoma cell line

  • Authors:
    • Fazhen Bao
    • Jie Qiu
    • Hong Zhang
  • View Affiliations

  • Published online on: April 26, 2012     https://doi.org/10.3892/mmr.2012.891
  • Pages: 185-190
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Abstract

β-elemene is extracted from the Chinese medicinal herb Curcuma Wenyujin. It has a broad-spectrum antitumor effect on many types of cancer. However, the exact mechanism of action of β-elemene in hepatocellular carcinoma (HCC) remains unknown. Histone H1 is thought to act as a repressor of transcription by promoting the compaction of chromatin into higher order structures. We found that histone H1 plays an important role in the antitumor function of β-elemene. In this study, histone H1 expression in the H22 murine hepatocellular carcinoma cell line was confirmed, with P388D1 cells serving as a positive control. Furthermore, H22 cells were cultured with β-elemene for different time-points in vitro and treated with different dose-dependent β-elemene in an experimental H22 HCC xenograft transplantation model to confirm whether β-elemene inhibited the growth of H22 tumor cells. In addition, measurements of histone H1 expression both in vitro and in vivo enabled us to demonstrate that β-elemene affects the expression of histone H1 only at the protein level, but is not involved in regulation at the gene level. Our results clearly show that the effect of β-elemene on inhibiting the growth of H22 tumor cells is time- and dose-dependent. In conclusion, β-elemene inhibits the growth of H22 cells by enhancing the expression of histone H1 only at the protein level. This finding may provide insight into a new mechanism of the antitumor action of β-elemene.

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July 2012
Volume 6 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
APA
Bao, F., Qiu, J., & Zhang, H. (2012). Potential role of β-elemene on histone H1 in the H22 ascites hepatoma cell line. Molecular Medicine Reports, 6, 185-190. https://doi.org/10.3892/mmr.2012.891
MLA
Bao, F., Qiu, J., Zhang, H."Potential role of β-elemene on histone H1 in the H22 ascites hepatoma cell line". Molecular Medicine Reports 6.1 (2012): 185-190.
Chicago
Bao, F., Qiu, J., Zhang, H."Potential role of β-elemene on histone H1 in the H22 ascites hepatoma cell line". Molecular Medicine Reports 6, no. 1 (2012): 185-190. https://doi.org/10.3892/mmr.2012.891