miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Ma,Wenqi; Yu,Qiang; Jiang,Jue; Du,Xiaopeng; Huang,Lili; Zhao,Linlin; Zhou,Qi

doi:10.3892/ol.2016.4269

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Authors:
- Wenqi Ma
- Qiang Yu
- Jue Jiang
- Xiaopeng Du
- Lili Huang
- Linlin Zhao
- Qi Zhou
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Affiliations: Department of Ultrasound, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
Published online on: February 24, 2016 https://doi.org/10.3892/ol.2016.4269
Pages: 2583-2589

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Abstract

Colorectal cancer (CRC) is a highly invasive tumor that is frequently associated with distant metastasis, which is the primary cause of poor prognosis. However, the mechanisms of metastasis remain poorly understood. MicroRNAs (miRNAs/miRs) have been considered to be implicated in CRC progression. In particular, miR-517a is proposed as a novel tumor-associated miRNA and has a potential role in tumor metastasis. The expression of miR-517a in CRC specimens was detected by reverse transcription‑quantitative polymerase chain reaction. Transwell assays were performed to determine the migration and invasion of CRC cells. The putative target genes of miR‑517a were disclosed using publicly available databases and western blot analysis. The present study identified that the expression of miR‑517a was significantly higher in CRC tissues as compared with adjacent non‑tumor tissues. Clinical analysis indicated that increased expression of miR-517a was correlated with poor prognostic features and poor long‑term survival of CRC patients. In vitro evidences demonstrated that downregulation of miR‑517a inhibited cell migration and invasion in HCT-116 cells. By contrast, upregulation of miR‑517a increased the number of migrated and invaded SW480 cells. Notably, miR-517a expression was inversely regulated by forkhead box J3 (FOXJ3) abundance in CRC cells. Furthermore, an inverse correlation between miR‑517a and FOXJ3 expression was observed in CRC tissues. In conclusion, miR-517a appears to be an independent prognostic marker for predicting survival of CRC patients, and may promote cell migration and invasion by inhibiting FOXJ3.

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