Open Access

Mechanism analysis of colorectal cancer according to the microRNA expression profile

  • Authors:
    • Hong Li
    • Huichao Zhang
    • Gang Lu
    • Qingjing Li
    • Jifeng Gu
    • Yuan Song
    • Shejun Gao
    • Yawen Ding
  • View Affiliations

  • Published online on: August 16, 2016     https://doi.org/10.3892/ol.2016.5027
  • Pages: 2329-2336
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to identify specific microRNAs (miRs) and their predicted target genes to clarify the molecular mechanisms of colorectal cancer (CRC). An miR expression profile (array ID, GSE39833), which consisted of 88 CRC samples with various tumor‑necrosis‑metastasis stages and 11 healthy controls, was downloaded from the Gene Expression Omnibus database. Subsequently, the differentially expressed miRs and their target genes were screened. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways of target genes were analyzed using the Database for Annotation Visualization and Integrated Discovery. A protein‑protein interaction (PPI) network of the target genes was constructed using the Search Tool for the Retrieval of Interacting Genes database. The present study identified a total of 18 differentially expressed miRs (upregulated, 8; downregulated, 10) in the sera of the CRC patients compared with the healthy controls. Of these, 3 upregulated (let‑7b, miR‑1290 and miR‑126) and 2 downregulated (miR‑16 and miR‑760) differentially expressed miRs and their target genes, including cyclin D1 (CCND1), v‑myc avian myelocytomatosis viral oncogene homolog (MYC), phosphoinositide‑3‑kinase, regulatory subunit 2 (beta) (PIK3R2) and SMAD family member 3 (SMAD3), were significantly enriched in the CRC developmental pathway. All these target genes had higher node degrees in the PPI network. In conclusion, let‑7b, miR‑1290, miR‑126, miR‑16 and miR‑760 and their target genes, CCND1, MYC, PIK3R2 and SMAD3, may be important in the molecular mechanisms for the progression of CRC.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Li H, Zhang H, Lu G, Li Q, Gu J, Song Y, Gao S and Ding Y: Mechanism analysis of colorectal cancer according to the microRNA expression profile. Oncol Lett 12: 2329-2336, 2016
APA
Li, H., Zhang, H., Lu, G., Li, Q., Gu, J., Song, Y. ... Ding, Y. (2016). Mechanism analysis of colorectal cancer according to the microRNA expression profile. Oncology Letters, 12, 2329-2336. https://doi.org/10.3892/ol.2016.5027
MLA
Li, H., Zhang, H., Lu, G., Li, Q., Gu, J., Song, Y., Gao, S., Ding, Y."Mechanism analysis of colorectal cancer according to the microRNA expression profile". Oncology Letters 12.4 (2016): 2329-2336.
Chicago
Li, H., Zhang, H., Lu, G., Li, Q., Gu, J., Song, Y., Gao, S., Ding, Y."Mechanism analysis of colorectal cancer according to the microRNA expression profile". Oncology Letters 12, no. 4 (2016): 2329-2336. https://doi.org/10.3892/ol.2016.5027