Open Access

Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels

  • Authors:
    • Giovanni Barillari
    • Clelia Palladino
    • Ilaria Bacigalupo
    • Patrizia Leone
    • Mario Falchi
    • Barbara Ensoli
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  • Published online on: July 29, 2016     https://doi.org/10.3892/ol.2016.4921
  • Pages: 2389-2394
  • Copyright: © Barillari et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The infection of uterine cervical epithelial cells by oncogenic, high-risk human papilloma viruses (HR‑HPVs) may lead to the development of cervical carcinoma. Of note, the incidence of this tumor is significantly increased in women infected by both HR‑HPV and human immunodeficiency virus (HIV)‑1. In this regard, previous studies have linked the HIV‑1 Tat protein, a trans‑activator of viral gene expression, to the pathogenesis of HIV‑associated malignancies. In particular, it has been shown that upon its release by acutely infected cells, Tat protein can enter human cells, thus modifying their phenotype. Based on these findings, the present study evaluated whether extracellular Tat protein could be taken up by human uterine cervical carcinoma cells, and whether this could affect the expression of HPV (E6 or E7) or cellular (p16 or p53) molecules, which are key to cervical carcinoma development or progression. The results indicated that extracellular, biologically active HIV‑1 Tat protein is taken up by human uterine cervical carcinoma cells, and that this is followed by an increase in the expression of the E6 protein of HPV, and by a reduction in the protein levels of the cellular oncosuppressor p53. Since p53 loss is associated with cell dedifferentiation and immortalization, these findings suggest a possible link between extracellular Tat protein and the high incidence and clinical aggressiveness of uterine cervical carcinoma observed in HIV/HPV doubly infected women.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Barillari G, Palladino C, Bacigalupo I, Leone P, Falchi M and Ensoli B: Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels. Oncol Lett 12: 2389-2394, 2016.
APA
Barillari, G., Palladino, C., Bacigalupo, I., Leone, P., Falchi, M., & Ensoli, B. (2016). Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels. Oncology Letters, 12, 2389-2394. https://doi.org/10.3892/ol.2016.4921
MLA
Barillari, G., Palladino, C., Bacigalupo, I., Leone, P., Falchi, M., Ensoli, B."Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels". Oncology Letters 12.4 (2016): 2389-2394.
Chicago
Barillari, G., Palladino, C., Bacigalupo, I., Leone, P., Falchi, M., Ensoli, B."Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels". Oncology Letters 12, no. 4 (2016): 2389-2394. https://doi.org/10.3892/ol.2016.4921