Investigation of the association between mitochondrial DNA and p53 gene mutations in transitional cell carcinoma of the bladder

Avcilar,Tuba; Kirac,Deniz; Ergec,Deniz; Koc,Gulsah; Ulucan,Korkut; Kaya,Zehra; Kaspar,Elif Cigdem; Turkeri,Levent; Guney,Ahmet Ilter

doi:10.3892/ol.2016.5000

Investigation of the association between mitochondrial DNA and p53 gene mutations in transitional cell carcinoma of the bladder

Authors:
- Tuba Avcilar
- Deniz Kirac
- Deniz Ergec
- Gulsah Koc
- Korkut Ulucan
- Zehra Kaya
- Elif Cigdem Kaspar
- Levent Turkeri
- Ahmet Ilter Guney
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Affiliations: Department of Medical Genetics, Faculty of Medicine, Marmara University, Istanbul 34890, Turkey, Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul 34755, Turkey, Department of Medical Biology, Faculty of Medicine, Istanbul Aydın University, Istanbul 34295, Turkey, Department of Medical Biology and Genetics, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkey, Department of Medical Biology, Faculty of Medicine, Yuzuncu Yıl University, Van 65080, Turkey, Department of Biostatistics, Faculty of Medicine, Yeditepe University, Istanbul 34755, Turkey, Department of Urology, Faculty of Medicine, Marmara University, Istanbul 34890, Turkey
Published online on: August 11, 2016 https://doi.org/10.3892/ol.2016.5000
Pages: 2872-2879

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Abstract

Bladder carcinoma is the most common malignancy of the urinary tract. The major aim of the present study is to investigate the association between mitochondrial DNA (mtDNA) and p53 gene mutations in bladder carcinoma. A total of 30 patients with transitional cell carcinoma and 27 controls were recruited for the study. Bladder cancer tissues were obtained by radical cystectomy or transurethral resection. Genomic DNA was extracted from peripheral blood. mtDNA and p53 genes were amplified by polymerase chain reaction and sequenced directly. A total of 37 polymorphisms were identified, among which, 2 mutations were significant in the patient group, and 1 mutation was significant in the control group. Additionally, 5 different moderate positive correlations between mtDNA mutations and 3 different positive correlations between p53 gene and mtDNA mutations were detected. The high incidence of mtDNA and p53 gene mutations in bladder cancer suggests that these genes could be important in carcinogenesis.

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