Open Access

Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells

  • Authors:
    • Lei Zhuang
    • Florian Steinberg
    • Beat Trueb
  • View Affiliations

  • Published online on: October 12, 2016     https://doi.org/10.3892/ol.2016.5245
  • Pages: 4524-4530
  • Copyright: © Zhuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Fibroblast growth factor receptor-like 1 (FGFRL1) is a transmembrane receptor that interacts with heparin and FGF ligands. In contrast to the classical FGF receptors, FGFR1 to FGFR4, it does not appear to affect cell growth and proliferation. In the present study, an inducible gene expression system was utilized in combination with a xenograft tumor model to investigate the effects of FGFRL1 on cell adhesion and tumor formation. It was determined that recombinant FGFRL1 promotes the adhesion of HEK 293 Tet‑On® cells in vitro. Moreover, when such cells are induced to express FGFRL1ΔC they aggregate into huge clusters. If injected into nude mice, the cells form large tumors. Notably, this tumor growth is completely inhibited when the expression of FGFRL1 is induced. The forced expression of FGFRL1 in the tumor tissue may restore contact inhibition, thereby preventing growth of the cells in nude mice. The results of the present study demonstrate that FGFRL1 acts as a tumor suppressor similar to numerous other cell adhesion proteins. It is therefore likely that FGFRL1 functions as a regular cell‑cell adhesion protein.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Zhuang L, Steinberg F and Trueb B: Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells. Oncol Lett 12: 4524-4530, 2016.
APA
Zhuang, L., Steinberg, F., & Trueb, B. (2016). Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells. Oncology Letters, 12, 4524-4530. https://doi.org/10.3892/ol.2016.5245
MLA
Zhuang, L., Steinberg, F., Trueb, B."Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells". Oncology Letters 12.6 (2016): 4524-4530.
Chicago
Zhuang, L., Steinberg, F., Trueb, B."Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells". Oncology Letters 12, no. 6 (2016): 4524-4530. https://doi.org/10.3892/ol.2016.5245