IL-1β promotes proliferation and migration of gallbladder cancer cells via Twist activation

Guo,Runsheng; Qin,Yiyu; Shi,Peidong; Xie,Jinbi; Chou,Ming; Chen,Yueyu

doi:10.3892/ol.2016.5254

IL-1β promotes proliferation and migration of gallbladder cancer cells via Twist activation

Authors:
- Runsheng Guo
- Yiyu Qin
- Peidong Shi
- Jinbi Xie
- Ming Chou
- Yueyu Chen
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Affiliations: Department of General Surgery, Jiading Central Hospital, Shanghai 201800, P.R. China, Clinical College, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China, Department of Gastroenterology, Jiading Central Hospital, Shanghai 201899, P.R. China, Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 201705, P.R. China
Published online on: October 13, 2016 https://doi.org/10.3892/ol.2016.5254
Pages: 4749-4755

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Abstract

Increasing evidence has revealed a correlation between chronic inflammation and gallbladder cancer (GBC). However, the underlying molecular mechanisms remain to be elucidated. In the present study, secretion of interleukin (IL)‑1β was examined in tissues of GBC, chronic cholecystitis and normal gallbladder, as well as in the supernatant of GBC‑SD, SGC996 and HIBEpiC cells. The effect of IL‑1β on the proliferation and migration of GBC cell lines was also evaluated. In addition, the role of Twist in IL‑1β‑induced proliferation of GBC cells was also studied. It was observed that the level of IL‑1β protein in normal gallbladder tissue was low, while it was significantly increased in GBC and chronic cholecystitis tissues. The level of IL‑1β protein and mRNA in GBC‑SD and SGC996 cells was markedly higher than those in HIBEpiC cells. Exogenous IL‑1β promoted the proliferation of GBC‑SD and SGC996 cells in vitro and in vivo, and also promoted migration in vitro. The level of Twist protein was significantly increased following treatment with exogenous IL‑1β. In addition, gene silencing of Twist blocked IL‑1β‑induced proliferation and migration of GBC‑SD and SGC996 cells. Taken together, these results indicate that IL‑1β promotes proliferation and migration of GBC cells via Twist activation.

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