Xanthohumol inhibits proliferation of laryngeal squamous cell carcinoma

Li,Yan; Wang,Kai; Yin,Shankai; Zheng,Hongliang; Min,Daliu

doi:10.3892/ol.2016.5313

Xanthohumol inhibits proliferation of laryngeal squamous cell carcinoma

Authors:
- Yan Li
- Kai Wang
- Shankai Yin
- Hongliang Zheng
- Daliu Min
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Affiliations: Department of Otolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China, Department of Otolaryngology, Zhabei Central Hospital, Shanghai 200070, P.R. China, Department of Otorhinolaryngology‑Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Department of Oncology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
Published online on: October 25, 2016 https://doi.org/10.3892/ol.2016.5313
Pages: 5289-5294

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Abstract

Xanthohumol is a flavonoid compound that exhibits antioxidant and anticancer effects, and is used to treat atherosclerosis. The aim of the present study was to investigate the effect of xanthohumol on the cell proliferation of laryngeal squamous cell carcinoma and to understand the mechanism of its action. The effects of xanthohumol on the cell viability and apoptosis rate of laryngeal squamous cell carcinoma SCC4 cells were assessed by Annexin V‑fluorescein isothiocyanate/propidium iodide staining. In addition, the expression levels of pro‑apoptotic proteins, caspase‑3, caspase-8, caspase‑9, poly ADP ribose polymerase (PARP) p53 and apoptosis‑inducing factor (AIF), as well as anti‑apoptotic markers, B‑cell lymphoma 2 (Bcl‑2) and myeloid cell leukemia 1 (Mcl‑1), were analyzed by western blotting. The results revealed that treatment with 40 µM xanthohumol significantly inhibited the proliferation of SCC4 cells. Furthermore, xanthohumol treatment (40 µM) induced SCC4 cell apoptosis, as indicated by the significant increase in activity and expression of caspase‑3, caspase‑8, caspase‑9, PARP, p53 and AIF. By contrast, the protein expression of Bcl‑2 and Mcl‑1 was significantly decreased following treatment with 40 µM xanthohumol. Taken together, the results of the present study indicated that xanthohumol mediates growth suppression and apoptosis induction, which was mediated via the suppression of Bcl‑2 and Mcl‑1 and activation of PARP, p53 and AIF signaling pathways. Therefore, future studies that investigate xanthohumol as a potential therapeutic agent for laryngeal squamous cell carcinoma are required.

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