MicroRNA‑31 functions as an oncogenic microRNA in cutaneous squamous cell carcinoma cells by targeting RhoTBT1

Lin,Nengxing; Zhou,Yu; Lian,Xin; Tu,Yating

doi:10.3892/ol.2017.5554

MicroRNA‑31 functions as an oncogenic microRNA in cutaneous squamous cell carcinoma cells by targeting RhoTBT1

Authors:
- Nengxing Lin
- Yu Zhou
- Xin Lian
- Yating Tu
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Affiliations: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
Published online on: January 2, 2017 https://doi.org/10.3892/ol.2017.5554
Pages: 1078-1082
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cutaneous squamous cell carcinoma (cSCC) is a malignancy of epidermal keratinocytes that is responsible for ~20% of annual skin cancer‑associated mortalities. Accumulating evidence demonstrates that the dysregulation of micro (mi)RNAs serves a significant role in the tumorigenesis and progression of human cSCC. MicroRNA‑31 (miR‑31) is upregulated in cSCC and is involved in cSCC development. However, the underlying mechanism remains unclear. The present study demonstrated that miR‑31 is upregulated in the cSCC cell line, A‑431, and that miR‑31 expression contributes to the cell proliferation and invasion of cSCC. In addition, bioinformatics combined with dual luciferase reporter analysis was applied to determine that the tumor suppressor RhoTBT1 was a direct target of miR‑31. In addition, miR‑31 mimics reduced RhoBTB1 expression in A‑431 cells. The results of MTT and Transwell assays demonstrated that knockdown of RhoBTB1 by short interfering RNA induced cell proliferation and invasion in A‑431 cells. These results indicated that suppression of RhoBTB1 may be involved in cSCC tumorigenesis, which was directly affected by miR‑31. In conclusion, the present study provides evidence that miR‑31 acts as an oncogene through direct repression of RhoTBT1 expression in cSCC cancer, suggesting a potential application of miR‑31 in prognosis prediction and its therapeutic application in cSCC.

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