Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells

Zhou,Yun; Wang,Chaoqun; Liu,Xia; Wu,Chengjun; Yin,Haitao

doi:10.3892/ol.2017.5587

Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells

Authors:
- Yun Zhou
- Chaoqun Wang
- Xia Liu
- Chengjun Wu
- Haitao Yin
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Affiliations: Department of Radiation Oncology, Xuzhou Central Hospital, The Affiliated Xuzhou Hospital of Medical College of Southeast University, Xuzhou Clinical School of Xuzhou Medical College, Xuzhou, Jiangsu 221009, P.R. China, College of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610064, P.R. China, Department of Pathology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China
Published online on: January 11, 2017 https://doi.org/10.3892/ol.2017.5587
Pages: 1143-1148
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the radiosensitizing effects of homeobox (HOX) transcript antisense RNA (HOTAIR) long non-coding RNA on breast cancer tumor cells and examine the underlying mechanisms. Recombinant plasmid vectors containing HOTAIR gene were constructed and MDA-MB231 cells were transfected with these plasmids using liposomes. The cells were treated with radiation and cell apoptosis, proliferation, and double-stranded DNA breaks were examined. HOXD10, phosphorylated AKT (p-AKT) and p-BAD expression levels were measured using western blot analysis. The results showed a higher expression of HOTAIR in advanced tumor cells. HOTAIR efficiently enhanced radioresistance in MDA-MB231 breast cancer cells and accelerated proliferation through the Akt pathway by targeting HOXD10. In conclusion, the findings demonstrated that HOTAIR gene is a valid therapeutic target for the reversal of radiotherapy resistance in breast cancer.

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