Downregulation of PDCD4 by miR‑21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model

Yuan,Haixin; Xin,Shiyong; Huang,Yaoping; Bao,Yingfan; Jiang,Hao; Zhou,Liqing; Ren,Xiaoqiang; Li,Liang; Wang,Qian; Zhang,Jianguo

doi:10.3892/ol.2017.6605

Downregulation of PDCD4 by miR‑21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model

Authors:
- Haixin Yuan
- Shiyong Xin
- Yaoping Huang
- Yingfan Bao
- Hao Jiang
- Liqing Zhou
- Xiaoqiang Ren
- Liang Li
- Qian Wang
- Jianguo Zhang
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Affiliations: Department of Urology, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Medical School of Pingdingshan University, Pingdingshan, Henan 467000, P.R. China, Zhengzhou Shuqing Medical College, Zhengzhou, Henan 450000, P.R. China, Department of General Surgery, Wendeng Central Hospital of Weihai, Weihai, Shandong 264200, P.R. China, Department of Rheumatism Immunity, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
Published online on: July 18, 2017 https://doi.org/10.3892/ol.2017.6605
Pages: 3371-3378
Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Programmed cell death 4 (PDCD4) is known to suppress neoplastic transformation, cell proliferation and metastasis, and to be downregulated by microRNA‑21 (miR‑21) in renal cell carcinoma (RCC) cell lines and tissues. The aim of the present study was to investigate the roles of and association between PDCD4 and miR‑21 in a nude mouse renal cancer model. A total of 24 BALB/c male nude mice were randomly assigned into the following three groups: Negative control (NC; n=8), miR‑21 inhibitor (n=8) and miR‑21 mimic (n=8). Subsequently, renal cell adenocarcinoma 786‑O cells were subcutaneously transplanted into the armpits of the mice, which were then injected daily with NC small interfering (si)RNA, precursor‑miR‑21 (mimic) or anti‑miR‑21 (inhibitor). Tumors were removed from the mice and weighed 16 days following 786‑O cell transplantation. In addition, the expression of miR‑21 and PDCD4 mRNA in cancer tissues was analyzed using reverse transcription‑quantitative PCR. The expression of PDCD4 protein in cancer tissues was also examined using immunohistochemistry and western blotting. Furthermore, 786‑O cells were transfected with PDCD4 siRNA or NC siRNA, and the effects of silencing PDCD4 on tumor cell growth, proliferation and invasion were investigated using soft agar colony formation, EdU cell proliferation assay and Transwell migration and invasion assays. Another 16 BALB/c male nude mice were randomly assigned into two groups as follows: NC (n=8) and PDCD4 siRNA (n=8). The 786‑O cells were subcutaneously transplanted into the armpits of the mice, which were subsequently injected daily with NC siRNA or PDCD4 siRNA. The tumors were removed and weighed 16 days following transplantation. Compared with the NC group, tumor weight in the miR‑21 mimic group was significantly increased. By contrast, tumor weight in the miR‑21 inhibitor group was significantly decreased. Similar to the results observed in human renal cancer tissue and cell lines, miR‑21 expression in the nude mouse renal cancer models was significantly upregulated in the miR‑21 mimic group compared with the NC group, while it was significantly lower in the miR‑21 inhibitor group. Furthermore, there was a significant reduction in PDCD4 protein levels in the miR‑21 mimic group and a significant increase in the miR‑21 inhibitor group compared with the NC, whereas PDCD4 mRNA expression was not significantly altered. In the EdU proliferation assay, the mean percentage of new cells that incorporated EdU was 28.6% in the NC siRNA group and significantly increased to 44.7% in PDCD4 siRNA transfected cells. In the soft agar colony formation assay, Transwell and migration and invasion assays, a significant increase in colony formation, migration and invasion capacity in PDCD4 siRNA‑transfected cells was observed compared with the NC. Furthermore, compared with the NC group, tumor weight in the PDCD4 siRNA group was significantly increased. Similar to the results observed in human renal cancer tissue and cell lines, miR‑21 promoted cancer cell hyperplasia and proliferation, and post‑transcriptionally downregulated PDCD4 protein expression, in the nude mouse renal cancer model. The results of the present study and previous studies indicate that PDCD4 and miR‑21 serve an important role in renal cancer. Thus, increasing PDCD4 expression or inhibiting miR‑21 expression may constitute effective novel therapeutic strategies for the treatment of renal cancer.

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