Open Access

Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC‑803 cells

  • Authors:
    • Bin He
    • Wen Wei
    • Ji Liu
    • Yundan Xu
    • Gang Zhao
  • View Affiliations

  • Published online on: July 20, 2017     https://doi.org/10.3892/ol.2017.6627
  • Pages: 3387-3394
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Curcumin is an anticancer compound that exerts anti‑proliferative and apoptotic effects via multiple molecular targets. The purpose of the present study was to investigate the anticancer effects of curcumin in combination with 5‑fluorouracil plus cisplatin (FP) on the MGC‑803 human gastric cancer cell line. Following treatment with curcumin and/or FP for 24, 48 and 72 h, cell viability, cell cycle progression and the apoptosis rate were evaluated using an MTT assay, flow cytometry and dual acridine orange/ethidium bromide staining, respectively. In addition, colony formation, Transwell migration and caspase‑3/caspase‑8 activity assays were performed. The expression of the apoptosis regulator B‑cell lymphoma‑2 (Bcl‑2) and Bcl‑2‑associated X protein (Bax) were detected by western blotting analysis. Following treatment with curcumin and/or FP, cell viability, colony formation and cell migration were significantly reduced compared with the untreated control group. The rate of apoptosis, caspase‑3/caspase‑8 activity and the expression of Bax were significantly increased, whereas Bcl‑2 expression was significantly reduced following treatment with curcumin and/or FP, compared with the untreated control group. The efficacy of curcumin combined with low‑dose FP was significantly increased, compared with that of curcumin combined with high‑dose FP (P<0.05). Therefore, curcumin may enhance the anticancer effects of FP chemotherapy in MGC‑803 cells through the promotion of apoptosis via the caspase‑3/caspase‑8, Bcl‑2 and Bax signaling pathways. These results suggest that curcumin may serve as a synergistic drug with chemotherapy regimen FP for the treatment of gastric cancer.
View Figures
View References

Related Articles

Journal Cover

September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
He B, Wei W, Liu J, Xu Y and Zhao G: Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC‑803 cells. Oncol Lett 14: 3387-3394, 2017
APA
He, B., Wei, W., Liu, J., Xu, Y., & Zhao, G. (2017). Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC‑803 cells. Oncology Letters, 14, 3387-3394. https://doi.org/10.3892/ol.2017.6627
MLA
He, B., Wei, W., Liu, J., Xu, Y., Zhao, G."Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC‑803 cells". Oncology Letters 14.3 (2017): 3387-3394.
Chicago
He, B., Wei, W., Liu, J., Xu, Y., Zhao, G."Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC‑803 cells". Oncology Letters 14, no. 3 (2017): 3387-3394. https://doi.org/10.3892/ol.2017.6627