Inhibition of LHX2 by miR‑124 suppresses cellular migration and invasion in non‑small cell lung cancer

Yang,Qinghui; Wan,Liang; Xiao,Can; Hu,Haibo; Wang,Longqiang; Zhao,Jun; Lei,Zhe; Zhang,Hong‑Tao

doi:10.3892/ol.2017.6607

Inhibition of LHX2 by miR‑124 suppresses cellular migration and invasion in non‑small cell lung cancer

Authors:
- Qinghui Yang
- Liang Wan
- Can Xiao
- Haibo Hu
- Longqiang Wang
- Jun Zhao
- Zhe Lei
- Hong‑Tao Zhang
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Affiliations: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Stomatology, The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Cardiothoracic Surgery, Huai'an Second People's Hospital, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223200, P.R. China, Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, Jiangsu 215123, P.R. China
Published online on: July 18, 2017 https://doi.org/10.3892/ol.2017.6607
Pages: 3429-3436
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Downregulated microRNA (miR)‑124 is common in numerous types of cancer, including non‑small cell lung cancer (NSCLC). A previous study by the authors demonstrated that LIM‑homeobox domain 2 (LHX2) was upregulated and promoted cell growth in NSCLC. However, whether LHX2 affects the migratory and invasive abilities of NSCLC cells and the association of LHX2 with miR‑124 remains unclear. The present study revealed that miR‑124 expression was frequently decreased in human NSCLC cells and tissues and negatively correlated with LHX2 expression, which was increased in NSCLC cells and tissues. Furthermore, the transfection of miR‑124 mimic significantly inhibited endogenous expression of LHX2 mRNA and protein in A549 and H1299 cells, and miR‑124 inhibitor promoted LHX2 expression. Of note, overexpression of miR‑124 in A549 and H1299 cells attenuated cellular migratory and invasive abilities, and this was observed in LHX2‑silenced A549 and H1299 cells. Knockdown of miR‑124 augmented the migratory and invasive abilities in A549 and H1299 cells. The 3'‑untranslated region of LHX2 transcript has also been identified to be a putative target of miR‑124. Taken together, the results revealed that miR‑124 may inhibit migration and invasion by repressing LHX2 expression in NSCLC cells. The findings of the present study suggested that overexpression of miR‑124 or silencing of LHX2 may provide a therapeutic strategy for advanced NSCLC.

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