Open Access

Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9

  • Authors:
    • Yi Hu
    • Aiping Ma
    • Shan Lin
    • Yang Yang
    • Guolin Hong
  • View Affiliations

  • Published online on: September 26, 2017     https://doi.org/10.3892/ol.2017.7065
  • Pages:6471-6476
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

CC chemokine receptor 9 (CCR9) serves a role in the drug resistance and metastasis of tumors. In the present study, a peptide specifically bound to CCR9 was obtained and the effect on tumor cells was observed. A Ph.D.‑12 phage display peptide library was used to screen for peptides binding specifically to the second extracellular loop of CCR9. The ratios of the input and output of phage clones increased gradually following three rounds of biopanning. A total of 8 positive phage clones were identified from DNA analysis. A phage clone, C‑4, was identified which exhibited higher affinity and specificity for the second extracellular loop of CCR9 in vitro compared with other clones. A peptide (P1; VHWDFRQWWQPS) was identified which may inhibit the corresponding phage, C‑4, binding to the second extracellular loop of CCR9. Furthermore, P1 was able to bind specifically with MOLT4 cells which exhibit marked expression of CCR9. In addition, P1 promoted the apoptosis of MOLT4 cells induced by doxorubicin, and inhibited the migration of MOLT4 cells in the presence of chemokine (C‑C motif) ligand 25. It was suggested that decreased activity in the phosphorylation of protein kinase B in MOLT4 cells may be responsible for the inhibition. In conclusion, the peptide P1 derived from a screened phage is able to specifically bind to CCR9 and inhibit the activity of CCR9. It has potential use as an antagonist in the treatment of CCR9‑overexpressed carcinoma.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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Copy and paste a formatted citation
APA
Hu, Y., Ma, A., Lin, S., Yang, Y., & Hong, G. (2017). Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9. Oncology Letters, 14, 6471-6476. https://doi.org/10.3892/ol.2017.7065
MLA
Hu, Y., Ma, A., Lin, S., Yang, Y., Hong, G."Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9". Oncology Letters 14.6 (2017): 6471-6476.
Chicago
Hu, Y., Ma, A., Lin, S., Yang, Y., Hong, G."Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9". Oncology Letters 14, no. 6 (2017): 6471-6476. https://doi.org/10.3892/ol.2017.7065