Open Access

Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1

  • Authors:
    • Qiang Wang
    • Shi‑Cheng Hu
    • Chun‑Sheng Yang
    • Jia‑Cun Chen
    • Jun‑Nian Zheng
    • Xiao‑Qing Sun
    • Jun‑Qi Wang
  • View Affiliations

  • Published online on: September 20, 2017     https://doi.org/10.3892/ol.2017.7006
  • Pages:6592-6596
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Despite previous advances, the treatment options for prostate cancer remain limited. For the purposes of gene knockdown, the utility of RNA interference has been demonstrated and is considered to have therapeutic potential. In the present study, a short hairpin RNA (shRNA) was used to assess the effect of special AT‑rich sequence binding protein (SATB1) downregulation on the growth and metastatic potential of prostate cancer in xenograft nude mice. A plasmid carrying shRNA targeting SATB1, pSilencer‑SATB1‑shRNA, was successfully engineered. Using this plasmid, significant downregulation of SATB1 mRNA and protein expression in the DU145 prostate cancer cells was observed. pSilencer‑SATB1‑shRNA was demonstrated to be markedly efficacious against prostate cancer xenografts in nude mice. These results may lead to a novel method of improving gene therapy efficacy against prostate cancer via regulating the function of SATB1.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Wang, Q., Hu, S., Yang, C., Chen, J., Zheng, J., Sun, X., & Wang, J. (2017). Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1. Oncology Letters, 14, 6592-6596. https://doi.org/10.3892/ol.2017.7006
MLA
Wang, Q., Hu, S., Yang, C., Chen, J., Zheng, J., Sun, X., Wang, J."Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1". Oncology Letters 14.6 (2017): 6592-6596.
Chicago
Wang, Q., Hu, S., Yang, C., Chen, J., Zheng, J., Sun, X., Wang, J."Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1". Oncology Letters 14, no. 6 (2017): 6592-6596. https://doi.org/10.3892/ol.2017.7006