microRNA‑34a overexpression inhibits cell migration and invasion via regulating SIRT1 in hepatocellular carcinoma

  • Authors:
    • Jianhui Zhou
    • Wenying Zhou
    • Fangen Kong
    • Xiaoyu Xiao
    • Haoyu Kuang
    • Yingxian Zhu
  • View Affiliations

  • Published online on: September 28, 2017     https://doi.org/10.3892/ol.2017.7090
  • Pages:6950-6954
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Abstract

Hepatocellular carcinoma (HCC) remains one of the most common types of malignancy with high mortality and morbidity rates. Previous studies have suggested that microRNAs (miRs) serve pivotal functions in various types of tumor. The aim of the present study was to assess the association between miR‑34a expression and HCC cell migration and invasion, and the potential underlying mechanisms. The miR‑34a overexpression vector or scramble control was transfected into human Hep3B and Huh7 cell lines. Transwell assays, and Matrigel and wound healing assays were used to detect the effects of miR‑34a expression on HCC cell invasion and migration, respectively. The expression of miR‑34a and the mRNA expression of other associated proteins were detected using quantitative reverse transcription polymerase chain reaction, and protein levels were measured using western blot analysis. Compared with the control, miR‑34a expression was significantly downregulated in Hep3B and Huh7 cells, but this was reversed by the transfection with exogenous miR‑34a (P<0.01). The number of migrated or invaded cells was significantly reduced by the overexpression of miR‑34a in Hep3B or Huh7 cells (P<0.01). The expression of sirtuin 1 was upregulated, while the level of acetylate‑p53 was downregulated by overexpression of miR‑34a. Taken together, the results of the present study suggested that the overexpression of miR‑34a may have suppressed HCC metastasis via inhibited cell migration and invasion.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Zhou, J., Zhou, W., Kong, F., Xiao, X., Kuang, H., & Zhu, Y. (2017). microRNA‑34a overexpression inhibits cell migration and invasion via regulating SIRT1 in hepatocellular carcinoma. Oncology Letters, 14, 6950-6954. https://doi.org/10.3892/ol.2017.7090
MLA
Zhou, J., Zhou, W., Kong, F., Xiao, X., Kuang, H., Zhu, Y."microRNA‑34a overexpression inhibits cell migration and invasion via regulating SIRT1 in hepatocellular carcinoma". Oncology Letters 14.6 (2017): 6950-6954.
Chicago
Zhou, J., Zhou, W., Kong, F., Xiao, X., Kuang, H., Zhu, Y."microRNA‑34a overexpression inhibits cell migration and invasion via regulating SIRT1 in hepatocellular carcinoma". Oncology Letters 14, no. 6 (2017): 6950-6954. https://doi.org/10.3892/ol.2017.7090