Open Access

Identification of differentially expressed genes in oral squamous cell carcinoma TCA8113 cells

  • Authors:
    • Jun Wang
    • Lifeng Li
    • Lina Gao
    • Chao Guan
    • Kexin Su
    • Linlin Li
    • Wenping Luo
    • Hongying Chen
    • Ping Ji
  • View Affiliations

  • Published online on: September 29, 2017     https://doi.org/10.3892/ol.2017.7108
  • Pages:7055-7068
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: PDF 0 views | HTML 0 views
0

Abstract

Previous studies have demonstrated that cancer cells with increased levels of aldehyde dehydrogenase ‘bright’ activity (ALDHbr) exhibit stem cell properties compared with cells exhibiting decreased ALDH activity (ALDHlow). To screen possible biomarkers of cancer stem cells in tongue squamous cell carcinoma, ALDHbr and ALDHlow cells were isolated from the tongue squamous cell carcinoma TCA8113 cell line, and suppression subtractive hybridization was performed to identify differentially expressed genes in the two subpopulations. A total of 240 positive clones were randomly selected for sequencing and were functionally characterized using bioinformatical tools. The results of the present study identified the differential expression of 104 clones, 62 of which corresponded to known genes and 42 of which corresponded to unknown genes. Cluster analysis revealed that the known genes were involved in the regulation of the cell cycle and cell differentiation. In addition, analysis of 10 signaling pathways revealed that genes were markedly altered in the ALDHbr cell subpopulation. Additional study is required to identify the function that these genes serve in the biomolecular regulatory mechanisms of cancer stem cells and to assist in explaining the biological behavior of oral squamous cell carcinoma.

Related Articles

Journal Cover

December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wang, J., Li, L., Gao, L., Guan, C., Su, K., Li, L. ... Ji, P. (2017). Identification of differentially expressed genes in oral squamous cell carcinoma TCA8113 cells. Oncology Letters, 14, 7055-7068. https://doi.org/10.3892/ol.2017.7108
MLA
Wang, J., Li, L., Gao, L., Guan, C., Su, K., Li, L., Luo, W., Chen, H., Ji, P."Identification of differentially expressed genes in oral squamous cell carcinoma TCA8113 cells". Oncology Letters 14.6 (2017): 7055-7068.
Chicago
Wang, J., Li, L., Gao, L., Guan, C., Su, K., Li, L., Luo, W., Chen, H., Ji, P."Identification of differentially expressed genes in oral squamous cell carcinoma TCA8113 cells". Oncology Letters 14, no. 6 (2017): 7055-7068. https://doi.org/10.3892/ol.2017.7108