Open Access

Reactivity of thyroid papillary carcinoma cells to thyroid stimulating hormone-dominated endocrine therapy

  • Authors:
    • Yuqin Ma
    • Xia Zhang
    • Yutao Wang
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/ol.2017.7173
  • Pages:7405-7409
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study investigated the effect of thyroid stimulating hormone (TSH) on the proliferation of papillary thyroid carcinoma (PTC) cells and the therapeutic effect of levothyroxine sodium (TH). PTC cells (TPC-1) were cultured using 0.1, 1.0 and 10 U/l TSH and 10-2, 10-4 and 10-6 mol/l TH. After the appropriate concentration was screened, TPC-1 cells were further divided into control group, TSH group, TH group and TSH+TH group. The cell proliferation was detected via methyl thiazolyl tetrazolium (MTT) method, TPC-1 cell cycle was detected via flow cytometer, and the mRNA and protein expression of cyclin D1 were detected via real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Compared with control group, TSH significantly promoted the proliferation of TPC-1 cells (P<0.05 or P<0.01), obviously promoted the transition of TPC-1 cells from G1 phase to S phase (P<0.01) and remarkably increased the mRNA and protein expression of cyclin D1 (P<0.01); but TH had a significant inhibitory effect on these results of TSH (P<0.05 or P<0.01). TSH can promote the proliferation of PTC cells, and the appropriate complement of TH can inhibit its proliferation.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Ma, Y., Zhang, X., & Wang, Y. (2017). Reactivity of thyroid papillary carcinoma cells to thyroid stimulating hormone-dominated endocrine therapy. Oncology Letters, 14, 7405-7409. https://doi.org/10.3892/ol.2017.7173
MLA
Ma, Y., Zhang, X., Wang, Y."Reactivity of thyroid papillary carcinoma cells to thyroid stimulating hormone-dominated endocrine therapy". Oncology Letters 14.6 (2017): 7405-7409.
Chicago
Ma, Y., Zhang, X., Wang, Y."Reactivity of thyroid papillary carcinoma cells to thyroid stimulating hormone-dominated endocrine therapy". Oncology Letters 14, no. 6 (2017): 7405-7409. https://doi.org/10.3892/ol.2017.7173