Anti‑angiogenic effect of Livistona chinensis seed extract in vitro and in vivo

  • Authors:
    • Zhiyun Cao
    • Liangpu Zheng
    • Jinyan Zhao
    • Qunchuan Zhuang
    • Zhenfeng Hong
    • Wei Lin
  • View Affiliations

  • Published online on: September 27, 2017     https://doi.org/10.3892/ol.2017.7075
  • Pages:7565-7570
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Abstract

The present study aimed to detect the impact of the ethanol extract of the Livistona chinensis seed (EELC) on angiogenesis in human umbilical vein endothelial cells (HUVECs). A chorioallantoic membrane (CAM) assay was used to detect the anti‑angiogenic activity of EELC in vivo. In vitro, the effect of EELC on the proliferation, migration and angiogenesis of HUVECs was determined by an MTT assay, a wound healing assay and a tube formation assay, respectively. The vascular endothelial growth factor (VEGF)‑A and VEGF receptor (VEGFR)‑2 protein and mRNA level were measured with ELISA and reverse transcription‑semi‑quantitative polymerase chain reaction. It was observed that EELC significantly decreased the formation of new vessels in the CAM assay. EELC inhibited the proliferation and migration of HUVECs. The extent of tube formation by HUVECs was also reduced by EELC. In addition, EELC treatment reduced the level of VEGF‑A and VEGFR‑2 mRNA and protein. The results suggest that EELC inhibits tumor angiogenesis through inhibiting the proliferation and migration of HUVECs, and by downregulating VEGF and VEGFR.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Cao, Z., Zheng, L., Zhao, J., Zhuang, Q., Hong, Z., & Lin, W. (2017). Anti‑angiogenic effect of Livistona chinensis seed extract in vitro and in vivo. Oncology Letters, 14, 7565-7570. https://doi.org/10.3892/ol.2017.7075
MLA
Cao, Z., Zheng, L., Zhao, J., Zhuang, Q., Hong, Z., Lin, W."Anti‑angiogenic effect of Livistona chinensis seed extract in vitro and in vivo". Oncology Letters 14.6 (2017): 7565-7570.
Chicago
Cao, Z., Zheng, L., Zhao, J., Zhuang, Q., Hong, Z., Lin, W."Anti‑angiogenic effect of Livistona chinensis seed extract in vitro and in vivo". Oncology Letters 14, no. 6 (2017): 7565-7570. https://doi.org/10.3892/ol.2017.7075