miR‑203a suppresses cell proliferation by targeting E2F transcription factor 3 in human gastric cancer
- Huiqin Yang
- Lixia Wang
- Xiaoli Tang
- Wenmei Bai
Published online on: October 17, 2017
MicroRNAs (miRs) are a class of short non‑coding RNAs that serve an essential role in the tumorigenesis of gastric cancer (GC). MiR‑203a has been reported as a tumor repressor in various types of human cancer. In the present study, the function of miR‑203a on the proliferation of GC cells was investigated. Bioinformatics analyses revealed that miR‑203a targets the 3'‑untranslated region of E2F transcription factor 3 (E2F3) messenger RNA. A luciferase reporter assay and western blot analysis were performed to confirm whether E2F3 was a target of miR‑203a. The relative luciferase activity was decreased when overexpressing miR‑203a with E2F3‑wild type pmirGLO‑3'‑untranslated region vector, compared with the control group in HEK293 cells. Overexpression of miR‑203a suppressed cell proliferation and colony formation of SGC‑7901 and AGS GC cells. Inhibition of miR‑203a promoted the proliferation of GC cells. Collectively, the results indicated that miR‑203a may function as a tumor suppressor in GC by targeting E2F3.