Open Access

Hypoxia‑inducible factor 1 mediates intermittent hypoxia‑induced migration of human breast cancer MDA‑MB‑231 cells

  • Authors:
    • Litao Liu
    • Wenlan Liu
    • Lili Wang
    • Ting Zhu
    • Jianhua Zhong
    • Ni Xie
  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/ol.2017.7223
  • Pages:7715-7722
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Metastasis is the major cause of triple‑negative breast cancer (TNBC)‑associated mortality. Hypoxia promotes cancer cell migration and remote metastasis, which occur with hypoxia inducible factor 1α (HIF‑1α) stabilization and vimentin upregulation. However, the evolutionary dynamics that link the changes in HIF‑1α and vimentin levels under hypoxic conditions are not well understood. In the present study, the effects of intermittent hypoxia (IH), continuous hypoxia (CH) and normoxia on the migration and proliferation of human TNBC MDA‑MB‑231 cells were investigated. The results demonstrated that IH significantly increased the migration of MDA‑MB‑231 cells, and this effect was dependent on the number of cycles of hypoxia‑reoxygenation. Unexpectedly, IH significantly inhibited cell proliferation, while CH only caused such an effect if hypoxia extended for ≥3 days. IH and CH induced HIF‑1α protein accumulation and vimentin upregulation, with a greater effect observed in IH. Knockdown of HIF‑1α with siRNA abolished IH‑induced cell migration and vimentin upregulation. In summary, multiple cycles of hypoxia and reoxygenation have a more pronounced effect on the promotion of TNBC invasiveness than CH; HIF‑1α activation and downstream vimentin upregulation may account for this phenotypic change.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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Copy and paste a formatted citation
APA
Liu, L., Liu, W., Wang, L., Zhu, T., Zhong, J., & Xie, N. (2017). Hypoxia‑inducible factor 1 mediates intermittent hypoxia‑induced migration of human breast cancer MDA‑MB‑231 cells. Oncology Letters, 14, 7715-7722. https://doi.org/10.3892/ol.2017.7223
MLA
Liu, L., Liu, W., Wang, L., Zhu, T., Zhong, J., Xie, N."Hypoxia‑inducible factor 1 mediates intermittent hypoxia‑induced migration of human breast cancer MDA‑MB‑231 cells". Oncology Letters 14.6 (2017): 7715-7722.
Chicago
Liu, L., Liu, W., Wang, L., Zhu, T., Zhong, J., Xie, N."Hypoxia‑inducible factor 1 mediates intermittent hypoxia‑induced migration of human breast cancer MDA‑MB‑231 cells". Oncology Letters 14, no. 6 (2017): 7715-7722. https://doi.org/10.3892/ol.2017.7223