Open Access

ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β‑catenin phosphorylation inhibition

  • Authors:
    • Ling Lan
    • Spyros Basourakos
    • Dai Cui
    • Xuemei Zuo
    • Wei Deng
    • Lili Huo
    • Hailing Chen
    • Guoying Zhang
    • Lili Deng
    • Bingyin Shi
    • Yong Luo
  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/ol.2017.7225
  • Pages:7733-7738
  • Copyright: © Lan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

All-trans-retinoic acid (ATRA) can enhance iodine uptake capability of thyroid tumors, but the mechanisms remain poorly understood. The aim of the present study was to investigate the effects of ATRA on isotope susceptibility, proliferation and invasion of anaplastic thyroid carcinoma (ATC) and potential mechanisms. SW1736 cells were treated with 1 µmol/l ATRA or 1% ethanol for 5 days. A cell line stably expressing β‑catenin‑shRNA was established. An iodine uptake assay was performed using 125I. Proliferation and invasiveness were tested using MTT and Transwell assays, respectively. Western blotting was used to assess the expression of β‑catenin, glycogen synthase kinase‑3β (GSK‑3β), sodium/iodine symporter (NIS) and proteins involved in epithelial‑mesenchymal transition. Cells pretreated with ATRA were injected subcutaneously into SCID mice. Mice were intraperitoneally injected with 131I once on the first day of treatment, and tumor growth was then assessed. After 35 days of 131I treatment, ATRA‑pretreated tumor volume and weight were decreased compared with the 131I alone group (163.32±19.57 vs. 332.06±21.37 mm3; 0.35±0.14 vs. 0.67±0.23 g, both P<0.05). Similar results were observed in the β‑catenin shRNA‑pretreated tumors. ATRA also increased the uptake of iodine by SW1736 cells (P<0.01), and similar results were observed in β‑catenin shRNA cells. ATRA treatment decreased the cell proliferation and invasion compared with control cells (all P<0.05), similar to β‑catenin shRNA. ATRA treatment decreased the expression of phosphorylated (p‑)β‑catenin, p‑GSK‑3β, vimentin, and fibronectin, and increased the expression of NIS and E‑cadherin, compared with the control. ATRA increased the iodine uptake and inhibited the proliferation and invasion of SW1736 cells, involving β‑catenin phosphorylation. In conclusion, ATRA could be used to improve the isotope sensitivity of ATC.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Lan, L., Basourakos, S., Cui, D., Zuo, X., Deng, W., Huo, L. ... Luo, Y. (2017). ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β‑catenin phosphorylation inhibition. Oncology Letters, 14, 7733-7738. https://doi.org/10.3892/ol.2017.7225
MLA
Lan, L., Basourakos, S., Cui, D., Zuo, X., Deng, W., Huo, L., Chen, H., Zhang, G., Deng, L., Shi, B., Luo, Y."ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β‑catenin phosphorylation inhibition". Oncology Letters 14.6 (2017): 7733-7738.
Chicago
Lan, L., Basourakos, S., Cui, D., Zuo, X., Deng, W., Huo, L., Chen, H., Zhang, G., Deng, L., Shi, B., Luo, Y."ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β‑catenin phosphorylation inhibition". Oncology Letters 14, no. 6 (2017): 7733-7738. https://doi.org/10.3892/ol.2017.7225