In vivo and in vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Jin,Shao‑Ju; Yang,Yun; Ma,Lei; Ma,Ben‑Hui; Ren,Li‑Ping; Guo,Liu‑Cheng; Wang,Wen‑Bao; Zhang,Yan‑Xin; Zhao,Zhi‑Jun; Cui,Mingchen

doi:10.3892/ol.2017.7227

In vivo and in vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Authors:
- Shao‑Ju Jin
- Yun Yang
- Lei Ma
- Ben‑Hui Ma
- Li‑Ping Ren
- Liu‑Cheng Guo
- Wen‑Bao Wang
- Yan‑Xin Zhang
- Zhi‑Jun Zhao
- Mingchen Cui
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Affiliations: Department of Pharmacology, The First Affiliated Hospital, Luohe Medical College, Luohe, Henan 462002, P.R. China, Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Emergency, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
Published online on: October 19, 2017 https://doi.org/10.3892/ol.2017.7227
Pages: 8000-8006
Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxysophoridine (OSR) is a major active alkaloid extracted from Sophoraalopecuroides L. The aim of the present study was to investigate the induction of the apoptotic effects of OSR on colorectal cancer cells in vivo and in vitro. The results of the MTT and colony formation assays demonstrated that the proliferation of HCT116 cells was inhibited by OSR in vitro. The characteristics of cellular apoptosis in OSR‑treated HCT116 cells were analyzed by Hoechst 33258 staining. It was also observed that the expression of caspase‑3, B‑cell lymphoma‑2 (Bcl‑2) associated X protein (Bax) and cytochrome c increased significantly upon OSR treatment. However, the expression of Bcl‑2 and poly ADP‑ribose polymerase‑1 (PARP‑1) was downregulated in OSR‑treated cells compared with untreated cells. The in vivo experiments identified that OSR significantly inhibited the growth of the transplanted mouse CT26 tumor tissue, upregulated the expression of caspase‑3, Bax and cytochrome c and downregulated the expression of Bcl‑2 and PARP‑1, as detected by reverse transcription‑quantitative polymerase chain reaction and western blotting. It may be concluded that OSR significantly induced apoptotic effects on colorectal cancer cells in vivo and in vitro, and that its mechanism may be associated with the Bcl‑2/Bax/caspase‑3 signaling pathway.

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