Open Access

Phosphorylation of phosphatase and tensin homolog induced by Helicobacter pylori promotes cell invasion by activation of focal adhesion kinase

  • Authors:
    • Zhen Yang
    • Ximei Cao
    • Wenting Xu
    • Chuan Xie
    • Jiang Chen
    • Yin Zhu
    • Nonghua Lu
  • View Affiliations

  • Published online on: November 16, 2017     https://doi.org/10.3892/ol.2017.7430
  • Pages:1051-1057
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Phosphorylation of the phosphatase and tensin homolog (PTEN) tumor suppressor at Ser380/Thr382/Thr383 residues is a novel mechanism underlying PTEN inactivation in gastric carcinogenesis, which may be triggered by Helicobacter pylori infection. To investigate this further, the effect of H. pylori infection on PTEN phosphorylation and the subsequent activation of focal adhesion kinase (FAK), were evaluated in gastric tissue samples from Mongolian gerbils and in the human gastric epithelial mucosa cell line GES‑1 using immunohistochemistry, western blotting and Transwell assays. The in vivo and in vitro results of the present study demonstrated that H. pylori infection induced the phosphorylation and inactivation of PTEN at Ser380/Thr382/383, and the subsequent phosphorylation and activation of FAK at Tyr397. Gastric epithelial cell invasion was also increased. Furthermore, stable expression of a dominant‑negative PTEN mutant inhibited the enhanced FAK activation and cell invasion induced by H. pylori infection. These results suggest that the mechanism underlying H. pylori‑induced carcinogenesis may involve promoting cell invasion through the phosphorylation of PTEN and the activation of FAK.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Yang, Z., Cao, X., Xu, W., Xie, C., Chen, J., Zhu, Y., & Lu, N. (2018). Phosphorylation of phosphatase and tensin homolog induced by Helicobacter pylori promotes cell invasion by activation of focal adhesion kinase. Oncology Letters, 15, 1051-1057. https://doi.org/10.3892/ol.2017.7430
MLA
Yang, Z., Cao, X., Xu, W., Xie, C., Chen, J., Zhu, Y., Lu, N."Phosphorylation of phosphatase and tensin homolog induced by Helicobacter pylori promotes cell invasion by activation of focal adhesion kinase". Oncology Letters 15.1 (2018): 1051-1057.
Chicago
Yang, Z., Cao, X., Xu, W., Xie, C., Chen, J., Zhu, Y., Lu, N."Phosphorylation of phosphatase and tensin homolog induced by Helicobacter pylori promotes cell invasion by activation of focal adhesion kinase". Oncology Letters 15, no. 1 (2018): 1051-1057. https://doi.org/10.3892/ol.2017.7430