Upregulation of microRNA‑383 inhibits the proliferation, migration and invasion of colon cancer cells
- Ying Cui
- Le‑Gao Chen
- Hai‑Bo Yao
- Jun Zhang
- Ke‑Feng Ding
Published online on: November 14, 2017
Copyright: © Cui et al.
This is an open access article distributed under the terms of Creative Commons Attribution License.
Increasing evidence demonstrates that microRNAs (miRNAs/miRs), a type of non‑coding small RNA, can regulate tumor cell migration, invasion and metastasis, and may therefore serve a major function in the occurrence and development of tumors. The present study investigated the effect of miR‑383 on the proliferation, migration and invasion of colon cancer HT‑29 and LoVo cell lines. The expression of miR‑383 in colon cancer and adjacent non‑tumor tissues was examined by reverse transcription‑quantitative polymerase chain reaction. MiR‑383 upregulation was stimulated by transfection with a miR‑383 mimic. Cell proliferation was measured with MTT and colony formation assays, and cell migration and invasion potential were examined by Transwell chamber assays. A proliferating‑inducing ligand (APRIL), myeloid cell leukemia‑1 and cyclooxygenase‑2 protein expression was analyzed by western blotting. The expression of miR‑383 was decreased in colon cancer tissues compared with adjacent non‑tumor tissues (P<0.05). Transfection with a miR‑383 mimic suppressed proliferation and inhibited cell migration and invasion in HT‑29 and LoVo colon cancer cell lines. Overexpression of miR‑383 in HT‑29 and LoVo cells resulted in the suppression of APRIL protein expression. In conclusion, miR‑383 was downregulated in colon cancer. The upregulation of miR‑383 inhibited proliferation, migration and invasion of colon cancer cells, potentially through the regulation of target gene APRIL.