Open Access

MicroRNA-20a promotes proliferation and invasion by directly targeting early growth response 2 in non‑small cell lung carcinoma

  • Authors:
    • Lai Wei
    • Fengming Ran
  • View Affiliations

  • Published online on: October 31, 2017     https://doi.org/10.3892/ol.2017.7299
  • Pages:271-277
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA‑20a (miR‑20a) serves a notable role in tumor development and progression; it functions differently in different types of malignant tumor, and its role and mechanism in non‑small cell lung carcinoma (NSCLC) remains unclear. In the present study, the effects of miR‑20a on the proliferation and invasion of NSCLC cells and the underlying mechanisms behind this were investigated. Reverse transcription‑quantitative polymerase chain reaction revealed that the expression level of miR‑20a was higher in human NSCLC than in normal tissues. Following this, the effect of miR‑20a on the proliferation, apoptosis, migration and invasion of NSCLCA‑549 cells was further evaluated. In vitro analysis, including a Cell Counting Kit‑8, colony formation and Transwell migration assay, indicated that miR‑20a‑knockdown inhibited the proliferation, invasion and migration, while promoting the cell apoptosis of the A‑549 cells. Early growth response 2 (EGR2) protein and mRNA levels were downregulated or upregulated following the overexpression or knockdown of miR‑20a, respectively. Dual‑luciferase reporter gene assays implied that EGR2 is a direct target gene of miR‑20a. The results of the present study indicated that miR‑20a may function as an oncomiR in the development of NSCLC by promoting cell viability and motility. The inhibition of miR‑20a could even become a novel therapeutic method for the treatment of NSCLC.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Wei, L., & Wei, L. (2018). MicroRNA-20a promotes proliferation and invasion by directly targeting early growth response 2 in non‑small cell lung carcinoma. Oncology Letters, 15, 271-277. https://doi.org/10.3892/ol.2017.7299
MLA
Wei, L., Ran, F."MicroRNA-20a promotes proliferation and invasion by directly targeting early growth response 2 in non‑small cell lung carcinoma". Oncology Letters 15.1 (2018): 271-277.
Chicago
Wei, L., Ran, F."MicroRNA-20a promotes proliferation and invasion by directly targeting early growth response 2 in non‑small cell lung carcinoma". Oncology Letters 15, no. 1 (2018): 271-277. https://doi.org/10.3892/ol.2017.7299