Open Access

Potential biological process of X‑linked inhibitor of apoptosis protein in renal cell carcinoma based upon differential protein expression analysis

  • Authors:
    • Chao Chen
    • Si Cong Zhao
    • Wen Zheng Yang
    • Zong Ping Chen
    • Yong Yan
  • View Affiliations

  • Published online on: November 9, 2017     https://doi.org/10.3892/ol.2017.7383
  • Pages:821-832
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The X‑linked inhibitor of apoptosis protein (XIAP) is the best characterized member of the IAP family and is a potent inhibitor of the caspase/apoptosis pathway. It has also been revealed that XIAP has additional biological functions that rely on its direct inhibition of apoptosis. In the present study, stably transfected Caki‑1 cells with XIAP‑knockdown were generated, and an isobaric tag for relative and absolute quantitation‑based proteomics approach was employed to investigate the regulatory mechanism of XIAP in renal cell carcinoma (RCC). The results demonstrate that the sensitivity of the RCC cell line to apoptotic stimulation increased markedly with XIAP‑knockdown. A number of differentially expressed proteins were detected between the original Caki‑1 cell line and the XIAP‑knockdown Caki‑1 cell line; 87 at 0 h (prior to etoposide treatment), 178 at 0.5 h and 169 at 3 h, while no differentially expressed proteins were detected (ratio >1.5 or <0.5; P<0.05) at 12 h after etoposide treatment. Through analysis of the differentially expressed proteins, it was revealed that XIAP may participate in the tumor protein p53 pathway, the Wnt signaling pathway, glucose metabolism, endoplasmic reticulum stress, cytoskeletal regulation and DNA repair. These results indicate that XIAP may have a number of biological functions and may provide an insight into the biomedical significance of XIAP overexpression in RCC.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Chen, C., Zhao, S., Yang, W., Chen, Z., & Yan, Y. (2018). Potential biological process of X‑linked inhibitor of apoptosis protein in renal cell carcinoma based upon differential protein expression analysis. Oncology Letters, 15, 821-832. https://doi.org/10.3892/ol.2017.7383
MLA
Chen, C., Zhao, S., Yang, W., Chen, Z., Yan, Y."Potential biological process of X‑linked inhibitor of apoptosis protein in renal cell carcinoma based upon differential protein expression analysis". Oncology Letters 15.1 (2018): 821-832.
Chicago
Chen, C., Zhao, S., Yang, W., Chen, Z., Yan, Y."Potential biological process of X‑linked inhibitor of apoptosis protein in renal cell carcinoma based upon differential protein expression analysis". Oncology Letters 15, no. 1 (2018): 821-832. https://doi.org/10.3892/ol.2017.7383