5-Fluorouracil may enrich cancer stem cells in canine mammary tumor cells in vitro

  • Authors:
    • Bin Zhou
    • Yipeng Jin
    • Di Zhang
    • Degui Lin
  • View Affiliations

  • Published online on: March 15, 2018     https://doi.org/10.3892/ol.2018.8267
  • Pages: 7987-7992
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Mammary gland carcinomas are the most common neoplasms in women and unsterilized female dogs. Owing to the existence of cancer stem cells (CSCs), chemotherapy is not able to cure these types of diseases completely. A number of studies have demonstrated that CSCs are resistant to chemotherapeutic drugs, but whether canine mammary tumor cells that have acquired resistance to 5‑fluorouracil (5‑FU) exhibited properties of CSCs remains unknown. The aim of the present study was to investigate whether 5‑fluorouracil‑resistant canine mammary tumor cells exhibited properties of CSCs. CSCs were analyzed using western blot assays, ultra‑low attachment sphere cultures, flow cytometry and migration (wound healing and Transwell) assays. The results indicated that, compared with parental cells, proteins associated with the Wnt/β‑catenin signaling pathway and aldehyde dehydrogenase 1 were overexpressed, the number and size of spheres in the 5‑FU‑resistant cells were increased, the ratio of CD44+/CD24‑/low cells was increased and the migratory ability was improved in vitro compared with the 5‑FU‑susceptible cells. In conclusion, stimulation with chemotherapeutic drugs including 5‑FU is a good method for increasing the proportion of canine mammary tumor stem cells in vitro, which may provide further understanding of chemotherapeutic methods and CSCs.

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhou B, Jin Y, Zhang D and Lin D: 5-Fluorouracil may enrich cancer stem cells in canine mammary tumor cells in vitro. Oncol Lett 15: 7987-7992, 2018.
APA
Zhou, B., Jin, Y., Zhang, D., & Lin, D. (2018). 5-Fluorouracil may enrich cancer stem cells in canine mammary tumor cells in vitro. Oncology Letters, 15, 7987-7992. https://doi.org/10.3892/ol.2018.8267
MLA
Zhou, B., Jin, Y., Zhang, D., Lin, D."5-Fluorouracil may enrich cancer stem cells in canine mammary tumor cells in vitro". Oncology Letters 15.5 (2018): 7987-7992.
Chicago
Zhou, B., Jin, Y., Zhang, D., Lin, D."5-Fluorouracil may enrich cancer stem cells in canine mammary tumor cells in vitro". Oncology Letters 15, no. 5 (2018): 7987-7992. https://doi.org/10.3892/ol.2018.8267