Open Access

Novel insights into biomarkers associated with renal cell carcinoma

  • Authors:
    • Binghai Chen
    • Zhimin Jiao
    • Xifeng Yin
    • Zhounan Qian
    • Jie Gu
    • Hao Sun
  • View Affiliations

  • Published online on: May 8, 2018     https://doi.org/10.3892/ol.2018.8665
  • Pages: 83-90
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Renal cell carcinoma (RCC) is a common form of cancer of the urinary tract. The present study aimed to identify driver genes in RCC using a bioinformatics approach. GSE53757 and GSE40435 microarray data were analyzed, and differentially expressed genes were filtered prior to gene ontology (GO) and pathway analysis. A protein‑protein interaction (PPI) network was established. Overall survival and recurrence were investigated and based on data presented in cBioPortal. The COPS7B gene within the PPI network was selected for further study in vitro. The present study identified 174 and 149 genes possessing a significant signal to noise ratio in GSE53757 and GSE40435, respectively. In total, 53 of these genes were selected based upon inclusion in both datasets. GO analysis indicated that PRKCDBP, EHD2, KCNJ10, ATP1A1, KCNJ1 and EHD2 may be involved in various biological processes. Furthermore, ALDH6A1, LDHA, SUCLG1 and ABAT may be involved in the propanoate metabolism pathway. A network consisting of 106 genes, and one typical cluster were constructed. In addition, COPS7B was selected, as it was associated with decreased overall survival and increased recurrence rates, in order to elucidate its function in RCC. Furthermore, upregulation of COPS7B was demonstrated to be predictive of advanced stage disease and metastasis of RCC. Finally, COPS7B‑knockdown inhibited RCC cell proliferation and invasion ability. Collectively, these results provided novel insights into COPS7B function, indicating that COPS7B may serve as a prognostic marker and therapeutic target in RCC.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Chen B, Jiao Z, Yin X, Qian Z, Gu J and Sun H: Novel insights into biomarkers associated with renal cell carcinoma. Oncol Lett 16: 83-90, 2018
APA
Chen, B., Jiao, Z., Yin, X., Qian, Z., Gu, J., & Sun, H. (2018). Novel insights into biomarkers associated with renal cell carcinoma. Oncology Letters, 16, 83-90. https://doi.org/10.3892/ol.2018.8665
MLA
Chen, B., Jiao, Z., Yin, X., Qian, Z., Gu, J., Sun, H."Novel insights into biomarkers associated with renal cell carcinoma". Oncology Letters 16.1 (2018): 83-90.
Chicago
Chen, B., Jiao, Z., Yin, X., Qian, Z., Gu, J., Sun, H."Novel insights into biomarkers associated with renal cell carcinoma". Oncology Letters 16, no. 1 (2018): 83-90. https://doi.org/10.3892/ol.2018.8665