Histone deacetylase inhibitor AR‑42 inhibits breast cancer cell growth and demonstrates a synergistic effect in combination with 5‑FU

  • Authors:
    • Ruihao Zhou
    • Juan Wu
    • Xiaofeng Tang
    • Xin Wei
    • Cheng Ju
    • Feifei Zhang
    • Jun Sun
    • Deyong Shuai
    • Zhiping Zhang
    • Qiong Liu
    • Xiao‑Bin Lv
  • View Affiliations

  • Published online on: May 31, 2018     https://doi.org/10.3892/ol.2018.8854
  • Pages: 1967-1974
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Abstract

AR‑42 is a member of a novelly discovered class of phenylbutyrate‑derived histone deacetylase inhibitors, and has a number of antitumor effects in a variety of tumor types; however, the role of AR‑42 and its possible mechanisms have not been reported in the treatment of breast cancer. The aim of the present study was to investigate the antitumor effects of AR‑42 and its associated mechanisms in breast cancer. MTT assays and colony formation assays were conducted to measure the proliferation of MCF‑7 cells, and flow cytometry was used to analyze cell apoptosis. The results revealed that AR‑42 induced cell apoptosis and suppressed cell growth in a dose‑ and time‑dependent manner. Mechanistically, AR‑42 treatment increased the acetylation of the p53 protein and prolonged the half‑life of the p53 protein; furthermore, AR‑42 treatment upregulated p21 and PUMA expression. Notably, AR‑42 had a synergistic effect on MCF‑7 cells in combination with fluorouracil, which is one of the most commonly used chemotherapeutic agents. In conclusion, the results indicated that AR‑42 inhibits breast cancer cell proliferation and induces apoptosis, indicating that AR‑42 is a potential therapeutic agent.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhou R, Wu J, Tang X, Wei X, Ju C, Zhang F, Sun J, Shuai D, Zhang Z, Liu Q, Liu Q, et al: Histone deacetylase inhibitor AR‑42 inhibits breast cancer cell growth and demonstrates a synergistic effect in combination with 5‑FU. Oncol Lett 16: 1967-1974, 2018
APA
Zhou, R., Wu, J., Tang, X., Wei, X., Ju, C., Zhang, F. ... Lv, X. (2018). Histone deacetylase inhibitor AR‑42 inhibits breast cancer cell growth and demonstrates a synergistic effect in combination with 5‑FU. Oncology Letters, 16, 1967-1974. https://doi.org/10.3892/ol.2018.8854
MLA
Zhou, R., Wu, J., Tang, X., Wei, X., Ju, C., Zhang, F., Sun, J., Shuai, D., Zhang, Z., Liu, Q., Lv, X."Histone deacetylase inhibitor AR‑42 inhibits breast cancer cell growth and demonstrates a synergistic effect in combination with 5‑FU". Oncology Letters 16.2 (2018): 1967-1974.
Chicago
Zhou, R., Wu, J., Tang, X., Wei, X., Ju, C., Zhang, F., Sun, J., Shuai, D., Zhang, Z., Liu, Q., Lv, X."Histone deacetylase inhibitor AR‑42 inhibits breast cancer cell growth and demonstrates a synergistic effect in combination with 5‑FU". Oncology Letters 16, no. 2 (2018): 1967-1974. https://doi.org/10.3892/ol.2018.8854