Clinical implications of hypoxia‑inducible factor‑1α and caveolin‑1 overexpression in isocitrate dehydrogenase‑wild type glioblastoma multiforme

Chen,Wenli; Cheng,Xing; Wang,Xiaobo; Wang,Jinshan; Wen,Xiaoling; Xie,Chaofan; Liao,Chuangxin

doi:10.3892/ol.2019.9929

Clinical implications of hypoxia‑inducible factor‑1α and caveolin‑1 overexpression in isocitrate dehydrogenase‑wild type glioblastoma multiforme

Authors:
- Wenli Chen
- Xing Cheng
- Xiaobo Wang
- Jinshan Wang
- Xiaoling Wen
- Chaofan Xie
- Chuangxin Liao
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Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Spine Surgery, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
Published online on: January 14, 2019 https://doi.org/10.3892/ol.2019.9929
Pages: 2867-2873
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma multiforme (GBM) is the most common type of primary brain tumour in adults, and presents a very low survival rate. Isocitrate dehydrogenase (IDH)1/2 mutations have been found in ~12% of glioblastomas and are associated with long‑term GBM survival. However, the risk factors that influence the prognosis of IDH‑wild type GBM remain unclear. Hypoxia‑inducible factor (HIF)‑1α, an important oxygen‑regulated transcription factor, has been demonstrated to serve a crucial role in tumour development and to be associated with a poor prognosis. In addition, caveolin‑1 (CAV1) is a plasma membrane organizing protein, the expression of which can also be regulated by a hypoxic microenvironment. The present study therefore aimed to examine the expression levels of HIF‑1α and CAV1, and their association with GBM prognosis. Reverse transcription‑quantitative polymerase chain reaction and western blotting were performed to analyse the expression levels of HIF‑1α and CAV1 in paired GBM tumour and adjacent non‑tumour tissues. Immunohistochemistry was used to analyse the expression of the two proteins in paraffin‑embedded tissues obtained from 42 patients with IDH‑wild type GBM. Statistical analyses were performed to examine the correlation between HIF‑1α and CAV1 expression and patient prognosis. The results revealed hat the expression levels of HIF‑1α and CAV1 were upregulated in IDH‑wild type GBM tissues compared to their paired non‑tumour tissues (P<0.001). The expression of CAV1 was significantly correlated with high HIF‑1α expression (P<0.01). In addition, overexpression of HIF‑1α and CAV1 was markedly associated with a poor prognosis (P<0.001). In conclusion, HIF‑1α and CAV1 may represent potential biomarkers for IDH‑wild type GBM prognosis and potential targets for the development of therapies extending GBM survival.

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