Open Access

Expression of bladder cancer‑associated glycans in murine tumor cell lines

  • Authors:
    • Marina Albertó
    • Hector Adrián Cuello
    • Cynthia Anabella Gulino
    • Marina Pifano
    • Denise Belgorosky
    • Mariano Rolando Gabri
    • Ana María Eiján
    • Valeria Inés Segatori
  • View Affiliations

  • Published online on: January 31, 2019     https://doi.org/10.3892/ol.2019.9995
  • Pages: 3141-3150
  • Copyright: © Albertó et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases. Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures. MB49 and MB49‑I are currently the only two murine cell lines available for the development of preclinical bladder cancer models. The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N‑glycolyl GM3 (NGcGM3) is a neo‑antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer. Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49‑I cells, in different growth conditions such as monolayer cultures, three‑dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors. The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines. STn was only identified in MB49 monolayers and spheroids. There are no reports concerning the expression of NGcGM3 in human or murine bladder cancer. In our hands, MB49 and MB49‑I expressed this ganglioside in all the growth conditions evaluated. The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology. The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models. To the best of our knowledge, the present study is the first to examine the expression of cancer‑associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
View Figures
View References

Related Articles

Journal Cover

March-2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Albertó M, Cuello HA, Gulino CA, Pifano M, Belgorosky D, Gabri MR, Eiján AM and Segatori VI: Expression of bladder cancer‑associated glycans in murine tumor cell lines. Oncol Lett 17: 3141-3150, 2019
APA
Albertó, M., Cuello, H.A., Gulino, C.A., Pifano, M., Belgorosky, D., Gabri, M.R. ... Segatori, V.I. (2019). Expression of bladder cancer‑associated glycans in murine tumor cell lines. Oncology Letters, 17, 3141-3150. https://doi.org/10.3892/ol.2019.9995
MLA
Albertó, M., Cuello, H. A., Gulino, C. A., Pifano, M., Belgorosky, D., Gabri, M. R., Eiján, A. M., Segatori, V. I."Expression of bladder cancer‑associated glycans in murine tumor cell lines". Oncology Letters 17.3 (2019): 3141-3150.
Chicago
Albertó, M., Cuello, H. A., Gulino, C. A., Pifano, M., Belgorosky, D., Gabri, M. R., Eiján, A. M., Segatori, V. I."Expression of bladder cancer‑associated glycans in murine tumor cell lines". Oncology Letters 17, no. 3 (2019): 3141-3150. https://doi.org/10.3892/ol.2019.9995