Modulation of P-glycoprotein expression by triptolide in adriamycin-resistant K562/A02 cells

  • Authors:
    • Hao Li
    • Lulu Hui
    • Wenlin Xu
    • Huiling Shen
    • Qiaoyun Chen
    • Lulu Long
    • Xiaolan Zhu
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  • Published online on: November 30, 2011     https://doi.org/10.3892/ol.2011.500
  • Pages: 485-489
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Abstract

Multidrug resistance is a serious obstacle encountered in leukemia treatment. Previous studies have found drug resistance in human leukemia is mainly associated with overexpression of the multidrug resistance gene 1 (MDR1). The aim of the present study was to investigate the modulation of P-glycoprotein expression by triptolide in adriamycin-resistant K562/A02 cells. The reverse effects of triptolide on drug resistance in K562/A02 cells were assessed by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The percentage of apoptotic cells was obtained from annexin V/fluorescein isothiocyanate (FITC) and propridium iodide (PI) double-staining. The effects of triptolide on P-glycoprotein activity were evaluated by measuring intracellular adriamycin accumulation. The expression of P-glycoprotein was determined by flow cytometry. A luciferase reporter gene assay was used to detect the transcriptional activity of the MDR1 promoter. Results revealed that triptolide decreased the degree of resistance of K562/A02 cells, and significantly inhibited P-glycoprotein expression and drug-transport function, and increased the accumulation of adriamycin in K562/A02 cells as measured by flow cytometry. A luciferase reporter gene assay demonstrated that triptolide was capable of inhibiting the transcriptional activity of the MDR1 promoter. Triptolide may effectively reverse the adriamycin resistance in K562/A02 cells via modulation of the P-glycoprotein expression and by increasing intracellular adriamycin accumulation.
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February 2012
Volume 3 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li H, Hui L, Xu W, Shen H, Chen Q, Long L and Zhu X: Modulation of P-glycoprotein expression by triptolide in adriamycin-resistant K562/A02 cells. Oncol Lett 3: 485-489, 2012
APA
Li, H., Hui, L., Xu, W., Shen, H., Chen, Q., Long, L., & Zhu, X. (2012). Modulation of P-glycoprotein expression by triptolide in adriamycin-resistant K562/A02 cells. Oncology Letters, 3, 485-489. https://doi.org/10.3892/ol.2011.500
MLA
Li, H., Hui, L., Xu, W., Shen, H., Chen, Q., Long, L., Zhu, X."Modulation of P-glycoprotein expression by triptolide in adriamycin-resistant K562/A02 cells". Oncology Letters 3.2 (2012): 485-489.
Chicago
Li, H., Hui, L., Xu, W., Shen, H., Chen, Q., Long, L., Zhu, X."Modulation of P-glycoprotein expression by triptolide in adriamycin-resistant K562/A02 cells". Oncology Letters 3, no. 2 (2012): 485-489. https://doi.org/10.3892/ol.2011.500