Expression of cell proliferating genes in patients with non-small cell lung cancer by immunohistochemistry and cDNA profiling

  • Authors:
    • Yongrong Mao
    • Jainping Wu
    • Sven Skog
    • Staffan Eriksson
    • Yiwei Zhao
    • Ji Zhou
    • Qimin He
  • View Affiliations

  • Published online on: May 1, 2005     https://doi.org/10.3892/or.13.5.837
  • Pages: 837-846
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Abstract

Thymidine kinase 1 (TK1) is a key enzyme involved in the synthesis of DNA precursors and thus, cell proliferation-dependent. Antibodies against TK1 have provided attractive tools for cancer diagnosis. Expression of TK1 in 158 non-small cell lung cancer (NSCLC) patients with 59 adenocarcinoma (AC) and 99 squamous cell carcinoma (SCC) was determined by anti-TK1 monoclonal antibody (mAb) 1E3 (AC, n=50; SCC, n=70). Parallel tumor sections were stained for Ki-67 (MIB-1), and TK1 expression was also investigated with anti-TK1 chicken IgY Ab (AC, n=9; SCC, n=29; normal lung tissues, n=10). In one AC and one SCC patient, gene profiling was done by cDNA array. Using the mAb 1E3, a significantly higher TK1 labeling index (LI) of AC patients was found (68%) compared to the LI of Ki-67 (36%). This difference was due to a significantly higher TK1 LI of tumor stage II and grade 2. Although no difference in the LI of TK1 and Ki-67 of SCC patients was found (54 vs. 53%), significantly higher TK1 LI of SCC patients of tumor grade 1 was found. Using the anti-TK1 IgY Ab, a higher TK1 LI of AC patients (78%) and SCC patients (66%) was found compared to staining with mAb 1E3 (68 vs. 54%), but it was not significantly different. Samples stained only for TK1 represented mostly tumor stages I and II and grades 1 and 2 of both AC and SCC. AC patients whose samples stained only for Ki-67 were found to be in stage I and grade 1. cDNA profiling showed that the expression of BRCA1, cyclin B1 and cdc2p34 was higher in AC compared to SCC, while the expression of IGFBP-3 and EGFR was higher in SCC. TK1 is apparently a more reliable marker in AC patients than Ki-67. However, a combination of the two markers may help identify patients of different stages and grades more efficiently, and cyclin/kinase complexes and growth factors/receptors may be useful markers in distinguishing AC from SCC.

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May 2005
Volume 13 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Mao Y, Wu J, Skog S, Eriksson S, Zhao Y, Zhou J and He Q: Expression of cell proliferating genes in patients with non-small cell lung cancer by immunohistochemistry and cDNA profiling. Oncol Rep 13: 837-846, 2005.
APA
Mao, Y., Wu, J., Skog, S., Eriksson, S., Zhao, Y., Zhou, J., & He, Q. (2005). Expression of cell proliferating genes in patients with non-small cell lung cancer by immunohistochemistry and cDNA profiling. Oncology Reports, 13, 837-846. https://doi.org/10.3892/or.13.5.837
MLA
Mao, Y., Wu, J., Skog, S., Eriksson, S., Zhao, Y., Zhou, J., He, Q."Expression of cell proliferating genes in patients with non-small cell lung cancer by immunohistochemistry and cDNA profiling". Oncology Reports 13.5 (2005): 837-846.
Chicago
Mao, Y., Wu, J., Skog, S., Eriksson, S., Zhao, Y., Zhou, J., He, Q."Expression of cell proliferating genes in patients with non-small cell lung cancer by immunohistochemistry and cDNA profiling". Oncology Reports 13, no. 5 (2005): 837-846. https://doi.org/10.3892/or.13.5.837