S100A4 expression with reduced E-cadherin expression predicts distant metastasis of human malignant melanoma cell lines in the NOD/SCID/γCnull (NOG) mouse model

  • Authors:
    • Norihiro Ikoma
    • Hitoshi Yamazaki
    • Yoshiyuki Abe
    • Yasuhisa Oida
    • Yasuyuki Ohnishi
    • Hiroshi Suemizu
    • Hozumi Matsumoto
    • Takashi Matsuyama
    • Yukinori Ohta
    • Akira Ozawa
    • Yoshito Ueyama
    • Masato Nakamura
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/or.14.3.633
  • Pages: 633-637
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Abstract

Tumor xenografts in immune-deficient mice (athymic nude mice and SCID mice) are well-established animal models for the study of human cancer. Several human melanoma cell lines were reported to metastasize in the immune-deficient mice models. However, metastatic rates were extremely low in spite of large numbers of injections of cancer cells, more than 1x106 cells/mouse. The NOD/SCID/γCnull (NOG) mouse shows multiple immunological dysfunctions, including cytokine production capability, in addition to the functional incompetence of T, B and natural killer (NK) cells. However, the immune-deficient mice, with preserved NK cell activity, might interfere with engraftment efficiency. We examined the distant metastasis of the human melanoma cell lines (A2058, A375, G361 and HMY-1, 1x104 cells/mouse) in the 6 weeks after intravenous inoculation. All four melanoma cell lines showed metastasis in the NOG mice, while no metastatic lesions were observed in the NOD/SCID mice. Metastatic lesions were noted in the liver and lung of 6/6 (100%) mice at A2058, 8/9 (89%) at A375, 2/6 (33%) at G361 and 2/8 (25%) at HMY-1. A2058 and A375 cell lines with high metastatic potentials show increased gene expression of S100A4. Western blot assay confirmed the increased protein levels of S100A4 in the A2058 and A375 cell lines. E-cadherin gene expression was conversely inhibited in these cell lines. The increased expression of S100A4 combined with inhibited E-cadherin expression resulted in high metastatic potentials of the human melanoma cell lines in vivo.

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September 2005
Volume 14 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Ikoma, N., Yamazaki, H., Abe, Y., Oida, Y., Ohnishi, Y., Suemizu, H. ... Nakamura, M. (2005). S100A4 expression with reduced E-cadherin expression predicts distant metastasis of human malignant melanoma cell lines in the NOD/SCID/γCnull (NOG) mouse model. Oncology Reports, 14, 633-637. https://doi.org/10.3892/or.14.3.633
MLA
Ikoma, N., Yamazaki, H., Abe, Y., Oida, Y., Ohnishi, Y., Suemizu, H., Matsumoto, H., Matsuyama, T., Ohta, Y., Ozawa, A., Ueyama, Y., Nakamura, M."S100A4 expression with reduced E-cadherin expression predicts distant metastasis of human malignant melanoma cell lines in the NOD/SCID/γCnull (NOG) mouse model". Oncology Reports 14.3 (2005): 633-637.
Chicago
Ikoma, N., Yamazaki, H., Abe, Y., Oida, Y., Ohnishi, Y., Suemizu, H., Matsumoto, H., Matsuyama, T., Ohta, Y., Ozawa, A., Ueyama, Y., Nakamura, M."S100A4 expression with reduced E-cadherin expression predicts distant metastasis of human malignant melanoma cell lines in the NOD/SCID/γCnull (NOG) mouse model". Oncology Reports 14, no. 3 (2005): 633-637. https://doi.org/10.3892/or.14.3.633