Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer

  • Authors:
    • Sun Y. Rha
    • Hei C. Jeung
    • Woo I. Yang
    • Jin J. Kim
    • Tae J. Oh
    • Sung W. An
    • Hyun C. Chung
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/or.15.4.749
  • Pages: 749-755
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Abstract

We analyzed hTERT splicing patterns with respect to telomerase activity in breast cancer. Using a cDNA microarray in 22 cell lines, we observed the difference in expression profiling based on the different levels of full-length variant expression with 71 selected genes. Using 33 known genes that act with the telomerase complex, we performed unsupervised clustering with all cell lines, and found a clustering tendency related to the full-length variant expression level. Using array-based CGH, highly altered genomic copy number changes were found more often in MCF-7 (159 genes) than in MDA-MB-231 (109 genes) and MDA-MB-435 (49 genes), suggesting more genomic changes in MCF-7 cells. On comparing MCF-7 with MDA-MB231 and MDA-MB-435 cell lines, we identified 8 genes with different copy numbers, including dystroglycan, which is located in the p12-21.2 area of chromosome 3. In conclusion, alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer, which require further study into their cause-and-effect relationship.

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April 2006
Volume 15 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Rha, S.Y., Jeung, H.C., Yang, W.I., Kim, J.J., Oh, T.J., An, S.W., & Chung, H.C. (2006). Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer. Oncology Reports, 15, 749-755. https://doi.org/10.3892/or.15.4.749
MLA
Rha, S. Y., Jeung, H. C., Yang, W. I., Kim, J. J., Oh, T. J., An, S. W., Chung, H. C."Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer". Oncology Reports 15.4 (2006): 749-755.
Chicago
Rha, S. Y., Jeung, H. C., Yang, W. I., Kim, J. J., Oh, T. J., An, S. W., Chung, H. C."Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer". Oncology Reports 15, no. 4 (2006): 749-755. https://doi.org/10.3892/or.15.4.749