Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler

  • Authors:
    • Hidefumi Sasaki
    • Katsuhiro Okuda
    • Osamu Kawano
    • Katsuhiko Endo
    • Haruhiro Yukiue
    • Tomoki Yokoyama
    • Motoki Yano
    • Yoshitaka Fujii
  • View Affiliations

  • Published online on: September 1, 2007     https://doi.org/10.3892/or.18.3.623
  • Pages: 623-628
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Abstract

Activating mutations of Ras gene families have been found in a variety of human malignancies, including lung cancer, suggesting their dominant role in tumorigenesis. Many studies have showed that the Kras gene is activated by point mutations in ≈15-20% of non-small cell lung cancers (NSCLCs), however, there are only a few reports on Nras mutations in NSCLC. We have genotyped Nras mutation status (n=195) and Kras mutation status (n=190) in surgically treated lung adenocarcinoma cases. The presence or absence of Nras and Kras mutations was analyzed by real-time quantitative polymerase chain reaction (PCR) with mutation-specific sensor and anchor probes. EGFR mutation status at kinase domain has already been reported. Nras mutation was found in 1 of 195 patients. This mutation was a G-to-T transversion, involving the substitution of the normal glycine (GGT) with cystein (TGT) and thought to be a somatic mutation. The patient was male and a smoker. Kras mutant patients (11.1%; 21/190) had a significantly worse prognosis than wild-type patients (p=0.0013). Eighty-two EGFR mutations at kinase domain had exclusively Nras or Kras mutations. Although Nras gene mutation might be one of the mechanisms of oncogenesis of lung adenocarcinoma, this was a very rare event. Further studies are needed to confirm the mechanisms of Nras mutations for the sensitivity of molecular target therapy for lung cancer.

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September 2007
Volume 18 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sasaki H, Okuda K, Kawano O, Endo K, Yukiue H, Yokoyama T, Yano M and Fujii Y: Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Oncol Rep 18: 623-628, 2007.
APA
Sasaki, H., Okuda, K., Kawano, O., Endo, K., Yukiue, H., Yokoyama, T. ... Fujii, Y. (2007). Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Oncology Reports, 18, 623-628. https://doi.org/10.3892/or.18.3.623
MLA
Sasaki, H., Okuda, K., Kawano, O., Endo, K., Yukiue, H., Yokoyama, T., Yano, M., Fujii, Y."Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler". Oncology Reports 18.3 (2007): 623-628.
Chicago
Sasaki, H., Okuda, K., Kawano, O., Endo, K., Yukiue, H., Yokoyama, T., Yano, M., Fujii, Y."Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler". Oncology Reports 18, no. 3 (2007): 623-628. https://doi.org/10.3892/or.18.3.623