CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines

  • Authors:
    • Young-Yim Kim
    • Chul-Kee Park
    • Seung-Ki Kim
    • Ji-Hoon Phi
    • Jin-Hyun Kim
    • Chae-Yong Kim
    • Kyu-Chang Wang
    • Byung-Kyu Cho
  • View Affiliations

  • Published online on: June 1, 2009     https://doi.org/10.3892/or_00000369
  • Pages: 1413-1419
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Abstract

CKD-602 7-[2-(N-isopropylamino)ethyl]-(20S)-camptothecin, belotecan) is a synthetic water-soluble camptothecin derivative and topoisomerase inhibitor that has been shown to have clinical anticancer effect against ovarian and lung cancer. We studied its anticancer effects on four human glioma cell lines, U87 MG, U343 MG, U251 MG and LN229. Cell viability was quantified by a modified 2-(2-methoxy-4-nitropheyl)-3-(4-nitropheyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt and significant time- and dose-dependent cytotoxicity was observed in all cell lines. Susceptibility to CKD-602 at 48 h after treatment varied among the four cell lines and their IC50 value was as follows: 9.07 nM (95% CI 0.18-37.42) for LN229, 14.57 nM (95% CI 0.86-47.33) for U251 MG, 29.13 nM (95% CI 0.35-101.23) for U343 MG, and 84.66 nM (95% CI 34.63-148.25) for U87 MG. CKD-602 induced cell cycle arrest at G2 phase and produced antiproliferative activity and apoptosis in all cell lines. Thus, CKD-602 showed a significant anticancer effect on glioma cells in vitro and is a promising candidate for further studies on malignant gliomas.

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June 2009
Volume 21 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Kim, Y., Park, C., Kim, S., Phi, J., Kim, J., Kim, C. ... Cho, B. (2009). CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines. Oncology Reports, 21, 1413-1419. https://doi.org/10.3892/or_00000369
MLA
Kim, Y., Park, C., Kim, S., Phi, J., Kim, J., Kim, C., Wang, K., Cho, B."CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines". Oncology Reports 21.6 (2009): 1413-1419.
Chicago
Kim, Y., Park, C., Kim, S., Phi, J., Kim, J., Kim, C., Wang, K., Cho, B."CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines". Oncology Reports 21, no. 6 (2009): 1413-1419. https://doi.org/10.3892/or_00000369